The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons

Mary Ann Manavalan, Vatsala Ruvini Jayasinghe, Rickinder Grewal, Krishna Moorthi Bhat

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Slit proteins act as repulsive axon guidance cues by activating receptors of the Roundabout (Robo) family. During early neurogenesis in Drosophila melanogaster, Slit prevents the growth cones of longitudinal tract neurons from inappropriately crossing the midline, thus restricting these cells to trajectories parallel to the midline. Slit is expressed in midline glial cells, and Robo is present in longitudinal axon tracts and growth cones. We showed that the enzyme Mummy (Mmy) controlled Slit-Robo signaling through mechanisms that affected both the ligand and the receptor. Mmy was required for the glycosylation of Slit, which was essential for Slit secretion. Mmy was also required for maintaining the abundance and spatial distribution of Robo through an indirect mechanism that was independent of Slit secretion. Moreover, secretion of Slit was required to maintain the fasciculation and position of longitudinal axon tracts, thus maintaining the hardwiring of the nervous system. Thus, Mmy is required for Slit secretion and for maintaining Robo abundance and distribution in the developing nervous system in Drosophila.

Original languageEnglish (US)
Article numbereaam5841
JournalScience Signaling
Volume10
Issue number484
DOIs
StatePublished - Jun 20 2017

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Mummies
Glycosylation
Axons
Maintenance
Neurology
Growth Cones
Cones
Nervous System
Fasciculation
Spatial distribution
Neurons
Neurogenesis
Drosophila melanogaster
Trajectories
Neuroglia
Drosophila
Ligands
Cues
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons. / Manavalan, Mary Ann; Jayasinghe, Vatsala Ruvini; Grewal, Rickinder; Bhat, Krishna Moorthi.

In: Science Signaling, Vol. 10, No. 484, eaam5841, 20.06.2017.

Research output: Contribution to journalArticle

Manavalan, Mary Ann ; Jayasinghe, Vatsala Ruvini ; Grewal, Rickinder ; Bhat, Krishna Moorthi. / The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons. In: Science Signaling. 2017 ; Vol. 10, No. 484.
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