The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2

John M. McPherson, Steven J. Sawamura, Yasushi Ogawa, Kelly Dineley, Pedro Carrillo, Karl A. Piez

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The growth inhibitory activity in conditioned medium of African green monkey kidney epithelial (BSC-1) cells that has been shown to arise, at least in part, from transforming growth factor β2 (TGF-β2) [Hanks, S. K., Armour, R., Baldwin, J. H., Maldonado, F., Spiess, J., & Holley, R. W. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 79-82] was tested for growth inhibitory activity prior to and following acidification. Similar to TGF-β1 from human platelets, the inhibitory activity from BSC-1 cells demonstrated an 8-10-fold stimulation following acidification, showing that the activity was secreted from the cells in latent form. Conditioned medium from BSC-1 cells was collected, acidified, and fractionated by procedures that separate TGF-β1 and -2. Biological activity was assayed by using the BSC-1 cell proliferation assay. Two active proteins with properties similar to known TGF-β1 and TGF-β2 were identified. Identity was confirmed by using immunological and amino acid sequencing techniques. These results were consistent with Northern blot analysis of total BSC-1 RNA, using cDNA probes for TGF-β1 and TGF-β2, which demonstrated strong signals for both mRNAs. Metabolic labeling in conjunction with two-dimensional gel electrophoresis revealed that the cells secrete approximately 10% TGF-β1 and 90% TGF-β2.

Original languageEnglish (US)
Pages (from-to)3442-3447
Number of pages6
JournalBiochemistry
Volume28
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Cercopithecus aethiops
Growth Inhibitors
Transforming Growth Factors
Acidification
Conditioned Culture Medium
Armor
Cell proliferation
Platelets
Bioactivity
Electrophoresis
Labeling
Protein Sequence Analysis
Electrophoresis, Gel, Two-Dimensional
Assays
Growth
Complementary DNA
Northern Blotting
Gels
RNA
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

McPherson, J. M., Sawamura, S. J., Ogawa, Y., Dineley, K., Carrillo, P., & Piez, K. A. (1989). The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2. Biochemistry, 28(8), 3442-3447.

The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2. / McPherson, John M.; Sawamura, Steven J.; Ogawa, Yasushi; Dineley, Kelly; Carrillo, Pedro; Piez, Karl A.

In: Biochemistry, Vol. 28, No. 8, 1989, p. 3442-3447.

Research output: Contribution to journalArticle

McPherson, JM, Sawamura, SJ, Ogawa, Y, Dineley, K, Carrillo, P & Piez, KA 1989, 'The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2', Biochemistry, vol. 28, no. 8, pp. 3442-3447.
McPherson, John M. ; Sawamura, Steven J. ; Ogawa, Yasushi ; Dineley, Kelly ; Carrillo, Pedro ; Piez, Karl A. / The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2. In: Biochemistry. 1989 ; Vol. 28, No. 8. pp. 3442-3447.
@article{df9cb376109d457593dd45328894a624,
title = "The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2",
abstract = "The growth inhibitory activity in conditioned medium of African green monkey kidney epithelial (BSC-1) cells that has been shown to arise, at least in part, from transforming growth factor β2 (TGF-β2) [Hanks, S. K., Armour, R., Baldwin, J. H., Maldonado, F., Spiess, J., & Holley, R. W. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 79-82] was tested for growth inhibitory activity prior to and following acidification. Similar to TGF-β1 from human platelets, the inhibitory activity from BSC-1 cells demonstrated an 8-10-fold stimulation following acidification, showing that the activity was secreted from the cells in latent form. Conditioned medium from BSC-1 cells was collected, acidified, and fractionated by procedures that separate TGF-β1 and -2. Biological activity was assayed by using the BSC-1 cell proliferation assay. Two active proteins with properties similar to known TGF-β1 and TGF-β2 were identified. Identity was confirmed by using immunological and amino acid sequencing techniques. These results were consistent with Northern blot analysis of total BSC-1 RNA, using cDNA probes for TGF-β1 and TGF-β2, which demonstrated strong signals for both mRNAs. Metabolic labeling in conjunction with two-dimensional gel electrophoresis revealed that the cells secrete approximately 10{\%} TGF-β1 and 90{\%} TGF-β2.",
author = "McPherson, {John M.} and Sawamura, {Steven J.} and Yasushi Ogawa and Kelly Dineley and Pedro Carrillo and Piez, {Karl A.}",
year = "1989",
language = "English (US)",
volume = "28",
pages = "3442--3447",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "8",

}

TY - JOUR

T1 - The growth inhibitor of African green monkey (BSC-1) cells is Transforming Growth Factors β1 and β2

AU - McPherson, John M.

AU - Sawamura, Steven J.

AU - Ogawa, Yasushi

AU - Dineley, Kelly

AU - Carrillo, Pedro

AU - Piez, Karl A.

PY - 1989

Y1 - 1989

N2 - The growth inhibitory activity in conditioned medium of African green monkey kidney epithelial (BSC-1) cells that has been shown to arise, at least in part, from transforming growth factor β2 (TGF-β2) [Hanks, S. K., Armour, R., Baldwin, J. H., Maldonado, F., Spiess, J., & Holley, R. W. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 79-82] was tested for growth inhibitory activity prior to and following acidification. Similar to TGF-β1 from human platelets, the inhibitory activity from BSC-1 cells demonstrated an 8-10-fold stimulation following acidification, showing that the activity was secreted from the cells in latent form. Conditioned medium from BSC-1 cells was collected, acidified, and fractionated by procedures that separate TGF-β1 and -2. Biological activity was assayed by using the BSC-1 cell proliferation assay. Two active proteins with properties similar to known TGF-β1 and TGF-β2 were identified. Identity was confirmed by using immunological and amino acid sequencing techniques. These results were consistent with Northern blot analysis of total BSC-1 RNA, using cDNA probes for TGF-β1 and TGF-β2, which demonstrated strong signals for both mRNAs. Metabolic labeling in conjunction with two-dimensional gel electrophoresis revealed that the cells secrete approximately 10% TGF-β1 and 90% TGF-β2.

AB - The growth inhibitory activity in conditioned medium of African green monkey kidney epithelial (BSC-1) cells that has been shown to arise, at least in part, from transforming growth factor β2 (TGF-β2) [Hanks, S. K., Armour, R., Baldwin, J. H., Maldonado, F., Spiess, J., & Holley, R. W. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 79-82] was tested for growth inhibitory activity prior to and following acidification. Similar to TGF-β1 from human platelets, the inhibitory activity from BSC-1 cells demonstrated an 8-10-fold stimulation following acidification, showing that the activity was secreted from the cells in latent form. Conditioned medium from BSC-1 cells was collected, acidified, and fractionated by procedures that separate TGF-β1 and -2. Biological activity was assayed by using the BSC-1 cell proliferation assay. Two active proteins with properties similar to known TGF-β1 and TGF-β2 were identified. Identity was confirmed by using immunological and amino acid sequencing techniques. These results were consistent with Northern blot analysis of total BSC-1 RNA, using cDNA probes for TGF-β1 and TGF-β2, which demonstrated strong signals for both mRNAs. Metabolic labeling in conjunction with two-dimensional gel electrophoresis revealed that the cells secrete approximately 10% TGF-β1 and 90% TGF-β2.

UR - http://www.scopus.com/inward/record.url?scp=0024566915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024566915&partnerID=8YFLogxK

M3 - Article

C2 - 2742846

AN - SCOPUS:0024566915

VL - 28

SP - 3442

EP - 3447

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 8

ER -