The haemophilus influenzae HMW1c protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin

Susan Grass, Cheryl F. Lichti, R. Reid Townsend, Julia Gross, Joseph W. St. Geme

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The Haemophilus influenzae HMW1 adhesin is a high-molecular weight protein that is secreted by the bacterial two-partner secretion pathway and mediates adherence to respiratory epithelium, an essential early step in the pathogenesis of H. influenzae disease. In recent work, we discovered that HMW1 is a glycoprotein and undergoes N-linked glycosylation at multiple asparagine residues with simple hexose units rather than N-acetylated hexose units, revealing an unusual Nglycosidic linkage and suggesting a new glycosyltransferase activity. Glycosylation protects HMW1 against premature degradation during the process of secretion and facilitates HMW1 tethering to the bacterial surface, a prerequisite for HMW1-mediated adherence. In the current study, we establish that the enzyme responsible for glycosylation of HMW1 is a protein called HMW1C, which is encoded by the hmw1 gene cluster and shares homology with a group of bacterial proteins that are generally associated with two-partner secretion systems. In addition, we demonstrate that HMW1C is capable of transferring glucose and galactose to HMW1 and is also able to generate hexose-hexose bonds. Our results define a new family of bacterial glycosyltransferases.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalPLoS Pathogens
Volume6
Issue number5
DOIs
StatePublished - May 2010
Externally publishedYes

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Glycosyltransferases
Hexoses
Asparagine
Haemophilus influenzae
Glycosylation
Secretory Pathway
Proteins
Respiratory Mucosa
Bacterial Proteins
Multigene Family
Galactose
Glycoproteins
Molecular Weight
Glucose
bacteria HMW1 protein
Enzymes

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

The haemophilus influenzae HMW1c protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin. / Grass, Susan; Lichti, Cheryl F.; Townsend, R. Reid; Gross, Julia; St. Geme, Joseph W.

In: PLoS Pathogens, Vol. 6, No. 5, 05.2010, p. 1-9.

Research output: Contribution to journalArticle

Grass, Susan ; Lichti, Cheryl F. ; Townsend, R. Reid ; Gross, Julia ; St. Geme, Joseph W. / The haemophilus influenzae HMW1c protein is a glycosyltransferase that transfers hexose residues to asparagine sites in the HMW1 adhesin. In: PLoS Pathogens. 2010 ; Vol. 6, No. 5. pp. 1-9.
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