The hepatitis C virus 3′-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase

Shelton Bradrick, Robert W. Walters, Matthias Gromeier

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Enhancement of eukaryotic messenger RNA (mRNA) translation initiation by the 3′ poly(A) tail is mediated through interaction of poly(A)-binding protein with eukaryotic initiation factor (eIF) 4G, bridging the 5′ terminal cap structure. In contrast to cellular mRNA, translation of the uncapped, non-polyadenylated hepatitis C virus (HCV) genome occurs independently of eIF4G and a role for 3′-untranslated sequences in modifying HCV gene expression is controversial. Utilizing cell-based and in vitro translation assays, we show that the HCV 3′-untranslated region (UTR) or a 3′ poly(A) tract of sufficient length interchangeably stimulate translation dependent upon the HCV internal ribosomal entry site (IRES). However, in contrast to cap-dependent translation, the rate of initiation at the HCV IRES was unaffected by 3′-untranslated sequences. Analysis of post-initiation events revealed that the 3′ poly(A) tract and HCV 3′-UTR improve translation efficiency by enabling termination and possibly ribosome recycling for successive rounds of translation.

Original languageEnglish (US)
Pages (from-to)1293-1303
Number of pages11
JournalNucleic Acids Research
Volume34
Issue number4
DOIs
StatePublished - Mar 2006
Externally publishedYes

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Poly A
3' Untranslated Regions
Hepacivirus
Messenger RNA
Eukaryotic Initiation Factor-4G
Poly(A)-Binding Proteins
Ribosomes
Genome
Gene Expression

ASJC Scopus subject areas

  • Genetics

Cite this

The hepatitis C virus 3′-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase. / Bradrick, Shelton; Walters, Robert W.; Gromeier, Matthias.

In: Nucleic Acids Research, Vol. 34, No. 4, 03.2006, p. 1293-1303.

Research output: Contribution to journalArticle

Bradrick, Shelton ; Walters, Robert W. ; Gromeier, Matthias. / The hepatitis C virus 3′-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase. In: Nucleic Acids Research. 2006 ; Vol. 34, No. 4. pp. 1293-1303.
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