The hexameric E. coli DnaB helicase can exist in different quarternary states

Xiong Yu, Maria J. Jezewska, Wlodzimierz Bujalowski, Edward H. Egelman

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The DnaB protein is the primary replicative helicase in Escherichia coli, and the active form of the protein is a hexamer. It has been reported that the protein forms a ring with strong 3-fold symmetry, which was suggested to be a trimer of dimers. We show that under different conditions, using either ATP, ATPγS, AMP-PNP or ADP as nucleotide cofactors, we always find two different forms of the DnaB ring; one with a 3-fold symmetry and one with 6-fold symmetry We have used scanning transmission electron microscopy for mass analysis, and have found that both forms are hexamers, excluding the possiblity that the 3-fold form is in fact a trimer of the 52 kDa monomer. We have also found rings that are in an intermediate state between these two. The existence of hexamers in discrete states shows that the transitions between these states must be cooperative. These observations suggest that there may be an equilibrium between two different conformations of the hexameric ring. The role of these two states in the mechanism of helicase action remains to be determined.

Original languageEnglish (US)
Pages (from-to)7-14
Number of pages8
JournalJournal of Molecular Biology
Volume259
Issue number1
DOIs
StatePublished - May 31 1996

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Adenylyl Imidodiphosphate
Scanning Transmission Electron Microscopy
Proteins
Adenosine Diphosphate
Nucleotides
Adenosine Triphosphate
Escherichia coli
E coli dnaB protein

Keywords

  • DNA replication
  • DnaB
  • Electron microscopy
  • Helicases
  • Image analysis

ASJC Scopus subject areas

  • Virology

Cite this

The hexameric E. coli DnaB helicase can exist in different quarternary states. / Yu, Xiong; Jezewska, Maria J.; Bujalowski, Wlodzimierz; Egelman, Edward H.

In: Journal of Molecular Biology, Vol. 259, No. 1, 31.05.1996, p. 7-14.

Research output: Contribution to journalArticle

Yu, Xiong ; Jezewska, Maria J. ; Bujalowski, Wlodzimierz ; Egelman, Edward H. / The hexameric E. coli DnaB helicase can exist in different quarternary states. In: Journal of Molecular Biology. 1996 ; Vol. 259, No. 1. pp. 7-14.
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