The host range phenotype displayed by a Sindbis virus glycoprotein variant results from virion aggregation and retention on the surface of mosquito cells

K. W. Boehme, Vsevolod Popov, H. W. Heidner

Research output: Contribution to journalArticle

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Abstract

The Sindbis virus variant NE2G216 is a PE2-containing host range mutant that is growth restricted in cultured mosquito cells (C6/36) due to inefficient release of virions from this cell type. The maturation defect of NE2G216 has been linked to the structures of N-linked oligosaccharides synthesized by arthropod cells. Analysis of C6/36 cells infected with NE2G216 by transmission electron microscopy revealed the presence of dense virus aggregates within cytoplasmic vacuoles and virus aggregates adhered to the cell surface. The virus aggregation phenotype of NE2G216 was reproduced in vertebrate cells (Pro-5) by the addition of 1-deoxymannojirimycin, an inhibitor of carbohydrate processing which limits the processing of N-linked oligosaccharides to structures that are structurally similar, albeit not identical, to those synthesized in C6/36 cells. We conclude that defective maturation of NE2G216 in mosquito cells is due to virion aggregation and retention on the cell surface and that this phenotype is directly linked to the carbohydrate-processing properties of these cells.

Original languageEnglish (US)
Pages (from-to)11398-11406
Number of pages9
JournalJournal of Virology
Volume74
Issue number23
DOIs
StatePublished - 2000
Externally publishedYes

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Sindbis virus
Sindbis Virus
Host Specificity
Culicidae
virion
host range
Virion
glycoproteins
Glycoproteins
Phenotype
phenotype
cells
Viruses
Oligosaccharides
cell aggregates
oligosaccharides
viruses
1-Deoxynojirimycin
Carbohydrates
carbohydrates

ASJC Scopus subject areas

  • Immunology

Cite this

The host range phenotype displayed by a Sindbis virus glycoprotein variant results from virion aggregation and retention on the surface of mosquito cells. / Boehme, K. W.; Popov, Vsevolod; Heidner, H. W.

In: Journal of Virology, Vol. 74, No. 23, 2000, p. 11398-11406.

Research output: Contribution to journalArticle

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