The human DINB1 gene encodes the DNA polymerase Polθ

Robert E. Johnson, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10-3 to 10-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.

Original languageEnglish (US)
Pages (from-to)3838-3843
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number8
DOIs
StatePublished - Apr 11 2000

Fingerprint

DNA-Directed DNA Polymerase
Genes
Pyrimidine Dimers
Thymine
DNA Damage
Escherichia coli
DNA
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The human DINB1 gene encodes the DNA polymerase Polθ. / Johnson, Robert E.; Prakash, Satya; Prakash, Louise.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 8, 11.04.2000, p. 3838-3843.

Research output: Contribution to journalArticle

@article{39165f841c744944beb84153720d3cfa,
title = "The human DINB1 gene encodes the DNA polymerase Polθ",
abstract = "The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10-3 to 10-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.",
author = "Johnson, {Robert E.} and Satya Prakash and Louise Prakash",
year = "2000",
month = "4",
day = "11",
doi = "10.1073/pnas.97.8.3838",
language = "English (US)",
volume = "97",
pages = "3838--3843",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "8",

}

TY - JOUR

T1 - The human DINB1 gene encodes the DNA polymerase Polθ

AU - Johnson, Robert E.

AU - Prakash, Satya

AU - Prakash, Louise

PY - 2000/4/11

Y1 - 2000/4/11

N2 - The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10-3 to 10-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.

AB - The human DINB1 gene shares a high degree of homology with the Escherichia coli dinB gene. Here, we purify the hDINB1-encoded protein and show that it is a DNA polymerase. Because hDinB1 is the eighth eukaryotic DNA polymerase to be described, we have named it DNA polymerase (Pol) θ. hPolθ is unable to bypass a cis-syn thymine-thymine dimer, nor does it bypass a (6- 4) photoproduct or an abasic site. We also examine the fidelity of hPolθ on nondamaged DNA templates by steady-state kinetic analyses and find that hPolθ misincorporates deoxynucleotides with a frequency of about 10-3 to 10-4. We discuss the relationship between the fidelity of hPolθ and its inability to bypass DNA damage.

UR - http://www.scopus.com/inward/record.url?scp=0034635953&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034635953&partnerID=8YFLogxK

U2 - 10.1073/pnas.97.8.3838

DO - 10.1073/pnas.97.8.3838

M3 - Article

VL - 97

SP - 3838

EP - 3843

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 8

ER -