The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression

Yau Jan Chyan, Susan Ackerman, Nancy S. Shepherd, O. Wesley McBride, Steven Widen, Samuel H. Wilson, Thomas Wood

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

DNA polymerase β (β-pol) is a single-copy gene that is considered to be part of the DNA repair machinery in mammalian cells. Using two human genomic libraries we have cloned the complete human β-pol gene and determined the organization of the β-pol coding sequence within the gene. The human β-pol gene spans 33 kb and contains 14 exons that range from 50 to 233 bp. The 13 introns vary from 96 bp to 6.5 kb. Information derived from this study was used in defining the location of a deletion/insertion type restriction fragment length polymorphism (RFLP) 5' to exon I of the human β-pol gene. This RFLP was utilized in linkage analysis of DNAs from CEPH families and the results confirm the previous assignment of the human β-pol gene to chromosome 8 (p12-p11). Analysis of mRNA from six human cell lines using the polymerase chain reaction showed the expression of two β-pol transcripts. Sequence analysis revealed that the size difference in these transcripts was due to deletion of the 58 bp sequence encoded by exon II, suggesting that the smaller transcript results from an alternative splicing of the exon II sequence during processing of the β-pol precursor RNA.

Original languageEnglish (US)
Pages (from-to)2719-2725
Number of pages7
JournalNucleic Acids Research
Volume22
Issue number14
StatePublished - Jul 25 1994

Fingerprint

Alternative Splicing
DNA-Directed DNA Polymerase
Gene expression
pol Genes
Gene Expression
DNA
Genes
Gene
Exons
Polymorphism
Restriction Fragment Length Polymorphisms
Deletion
Fragment
Cells
Restriction
Linkage Analysis
Chromosomes, Human, Pair 8
Polymerase Chain Reaction
Genomic Library
Polymerase chain reaction

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Chyan, Y. J., Ackerman, S., Shepherd, N. S., McBride, O. W., Widen, S., Wilson, S. H., & Wood, T. (1994). The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression. Nucleic Acids Research, 22(14), 2719-2725.

The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression. / Chyan, Yau Jan; Ackerman, Susan; Shepherd, Nancy S.; McBride, O. Wesley; Widen, Steven; Wilson, Samuel H.; Wood, Thomas.

In: Nucleic Acids Research, Vol. 22, No. 14, 25.07.1994, p. 2719-2725.

Research output: Contribution to journalArticle

Chyan, YJ, Ackerman, S, Shepherd, NS, McBride, OW, Widen, S, Wilson, SH & Wood, T 1994, 'The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression', Nucleic Acids Research, vol. 22, no. 14, pp. 2719-2725.
Chyan YJ, Ackerman S, Shepherd NS, McBride OW, Widen S, Wilson SH et al. The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression. Nucleic Acids Research. 1994 Jul 25;22(14):2719-2725.
Chyan, Yau Jan ; Ackerman, Susan ; Shepherd, Nancy S. ; McBride, O. Wesley ; Widen, Steven ; Wilson, Samuel H. ; Wood, Thomas. / The human DNA polymerase β gene structure. Evidence of alternative splicing in gene expression. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 14. pp. 2719-2725.
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