The IbeA invasin of adherent-invasive Escherichia coli mediates nteraction with intestinal epithelia and macrophages

Roberto J. Cieza, Jia Hu, Brittany N. Ross, Elena Sbrana, Alfredo Torres

Research output: Contribution to journalArticle

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Abstract

Adherent-invasive Escherichia coli (AIEC) pathogroup isolates are a group of isolates from the intestinal mucosa of Crohn's disease patients that can invade intestinal epithelial cells (IECs) or macrophages and survive and/or replicate within. We have identified the ibeA gene in the genome of AIEC strain NRG857c and report the contribution of IbeA to the interaction of AIEC with IECs and macrophages and colonization of the mouse intestine. An ibeA deletion mutant strain (NRG857cΔibeA) was constructed, and the in vitro effect on AIEC adhesion and invasion of nonpolarized and polarized Caco-2 cells, the adhesion and transcytosis of M-like cells, the intracellular survival in THP-1 macrophages, and the contribution to intestinal colonization of the CD-1 murine model of infection were evaluated. A significant reduction in invasion was observed with the ibeA mutant in Caco-2 and M-like cells, whereas adhesion was not affected. Complementation of the mutant reestablished Caco-2 invasive phenotype to wild-type levels. Reduction in invasion did not significantly affect transcytosis through M-like cells at early time points. The absence of ibeA significantly affected AIEC intramacrophage survival up to 24 h postinfection. No significant changes associated with IbeA were found in AIEC colonization across the murine gastrointestinal tract, but a slight reduction of gamma interferon was observed in the ceca of mice infected with the ibeA mutant. In addition, a decrease in the pathology scores was observed in the ilea and ceca of mice infected with the ibeA mutant. Our data support the function of IbeA in the AIEC invasion process, macrophage survival, and inflammatory response in the murine intestine.

Original languageEnglish (US)
Pages (from-to)1904-1918
Number of pages15
JournalInfection and Immunity
Volume83
Issue number5
DOIs
StatePublished - 2015

Fingerprint

Intestinal Mucosa
Macrophages
Escherichia coli
Transcytosis
Cecum
Cell Adhesion
Intestines
Epithelial Cells
Caco-2 Cells
Ileum
Crohn Disease
Interferon-gamma
Gastrointestinal Tract
Cell Survival
Genome
Pathology
Phenotype
Survival
Infection
Genes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

The IbeA invasin of adherent-invasive Escherichia coli mediates nteraction with intestinal epithelia and macrophages. / Cieza, Roberto J.; Hu, Jia; Ross, Brittany N.; Sbrana, Elena; Torres, Alfredo.

In: Infection and Immunity, Vol. 83, No. 5, 2015, p. 1904-1918.

Research output: Contribution to journalArticle

Cieza, Roberto J. ; Hu, Jia ; Ross, Brittany N. ; Sbrana, Elena ; Torres, Alfredo. / The IbeA invasin of adherent-invasive Escherichia coli mediates nteraction with intestinal epithelia and macrophages. In: Infection and Immunity. 2015 ; Vol. 83, No. 5. pp. 1904-1918.
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