The identification and characterization of a urinary mutagen resulting from cigarette smoke

Thomas H. Connor, V. M.Sadagopa Ramanujam, Jonathan B. Ward, Marvin S. Legator

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The urine of a cigarette smoker who excretes exceptionally mutageni curine was analyzed for several factors affecting mutagenicity. S. typhimurium strain TA98 was always more sensitive to XAD-2 urine concentrates than TA100. With TA98, as high as 85 revertants per ml of urine were produced. It was observed that incubation with β-glucuronidase was not required for expression of mutagenicity but that a complete S9 mix was needed to convert the material in the concentrate to the ultimate mutagenic species. TLC and HPLC separation of the XAD-2 urine concentrate resulted in the identification of trace amounts of the bladder carcinogen, 2-aminonapthalene (β-naphthylamine) and a considerable amount of a possible metabolite of 2-aminonaphthalene, 2-amino-7-naphthol. The identity of the compounds was confirmed by mass spectral analysis, and 2-amino-7-naphthol was shown to be a mutagen for TA100 and TA98 when activated by rat-liver S9.

Original languageEnglish (US)
Pages (from-to)161-172
Number of pages12
JournalMutation Research/Environmental Mutagenesis and Related Subjects
Volume113
Issue number2
DOIs
StatePublished - Apr 1983

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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