The immunomodulating v and W proteins of Nipah virus determine disease course

Benjamin A. Satterfield, Robert Cross, Karla A. Fenton, Krystle N. Agans, Christopher F. Basler, Thomas Geisbert, Chad Mire

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The viral determinants that contribute to Nipah virus (NiV)-mediated disease are poorly understood compared with other paramyxoviruses. Here we use recombinant NiVs (rNiVs) to examine the contributions of the NiV V and W proteins to NiV pathogenesis in a ferret model. We show that a V-deficient rNiV is susceptible to the innate immune response in vitro and behaves as a replicating non-lethal virus in vivo. Remarkably, rNiV lacking W expression results in a delayed and altered disease course with decreased respiratory disease and increased terminal neurological disease associated with altered in vitro inflammatory cytokine production. This study confirms the V protein as the major determinant of pathogenesis, also being the first in vivo study to show that the W protein modulates the inflammatory host immune response in a manner that determines the disease course.

Original languageEnglish (US)
Article number7483
JournalNature Communications
Volume6
DOIs
StatePublished - Jun 24 2015

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viruses
Virus Diseases
Nipah Virus
Viruses
proteins
pathogenesis
determinants
Proteins
respiratory diseases
Pulmonary diseases
Ferrets
Innate Immunity
Cytokines
Nipah virus W protein
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

The immunomodulating v and W proteins of Nipah virus determine disease course. / Satterfield, Benjamin A.; Cross, Robert; Fenton, Karla A.; Agans, Krystle N.; Basler, Christopher F.; Geisbert, Thomas; Mire, Chad.

In: Nature Communications, Vol. 6, 7483, 24.06.2015.

Research output: Contribution to journalArticle

Satterfield, Benjamin A. ; Cross, Robert ; Fenton, Karla A. ; Agans, Krystle N. ; Basler, Christopher F. ; Geisbert, Thomas ; Mire, Chad. / The immunomodulating v and W proteins of Nipah virus determine disease course. In: Nature Communications. 2015 ; Vol. 6.
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