Abstract
Critical-size bone defects usually require the use of autogenous bone grafts to heal. Harvesting of bone is traumatic, and results in high morbidity of donor site. A potential alternative to bone graft may be a bone substitute with adequate biocompatibility and biological properties produced from ceramics and/or bioresorbable or biodegradable polymers. In the present study, sheep osteoblasts isolated from cancellous bone chips were seeded and cultured for 1, 2, and 3 weeks on porous poly(L/DL-lactide) 80/20% scaffolds. The cell morphology was assessed from rhodamine staining and scanning electron microscopy, cell growth, and activity from the measurements of DNA, alkaline phosphatase activity, and total protein amount in the cell lysate, and mineral deposits from EDAX and van Kossa staining. The cells attached firmly to the scaffold walls, grew deeply into the pores, and exhibited morphology typical of osteoblasts. Mineralized noduli with a Ca/P ratio of 1.4 to 1.8 found in the scaffold indicated that the cells had maintained their osteoblastic phenotype. The amount of DNA, alkaline phosphatase activity, and the total amount of proteins increased with time of culturing, although at 3 weeks the cells did not yet reach the contact inhibition. These results demonstrate that three-dimensional porous poly(L/DL-lactide) 80/20% scaffolds provide a suitable substrate for the attachment and growth of primary osteoblasts, and might potentially be used as bone defect fillers and in the design of tissue-engineered bone substitutes.
| Original language | English |
|---|---|
| Pages (from-to) | 702-709 |
| Number of pages | 8 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 75 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2005 |
| Externally published | Yes |
Fingerprint
Keywords
- Bioresorbable scaffold
- Bone substitute
- Cell culture
- Osteoblasts
- Polylactide
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
Cite this
The in vitro growth and activity of sheep osteoblasts on three-dimensional scaffolds from poly(L/DL-lactide) 80/20%. / Gugala, Zbigniew; Gogolewski, Sylwester.
In: Journal of Biomedical Materials Research - Part A, Vol. 75, No. 3, 12.2005, p. 702-709.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The in vitro growth and activity of sheep osteoblasts on three-dimensional scaffolds from poly(L/DL-lactide) 80/20%
AU - Gugala, Zbigniew
AU - Gogolewski, Sylwester
PY - 2005/12
Y1 - 2005/12
N2 - Critical-size bone defects usually require the use of autogenous bone grafts to heal. Harvesting of bone is traumatic, and results in high morbidity of donor site. A potential alternative to bone graft may be a bone substitute with adequate biocompatibility and biological properties produced from ceramics and/or bioresorbable or biodegradable polymers. In the present study, sheep osteoblasts isolated from cancellous bone chips were seeded and cultured for 1, 2, and 3 weeks on porous poly(L/DL-lactide) 80/20% scaffolds. The cell morphology was assessed from rhodamine staining and scanning electron microscopy, cell growth, and activity from the measurements of DNA, alkaline phosphatase activity, and total protein amount in the cell lysate, and mineral deposits from EDAX and van Kossa staining. The cells attached firmly to the scaffold walls, grew deeply into the pores, and exhibited morphology typical of osteoblasts. Mineralized noduli with a Ca/P ratio of 1.4 to 1.8 found in the scaffold indicated that the cells had maintained their osteoblastic phenotype. The amount of DNA, alkaline phosphatase activity, and the total amount of proteins increased with time of culturing, although at 3 weeks the cells did not yet reach the contact inhibition. These results demonstrate that three-dimensional porous poly(L/DL-lactide) 80/20% scaffolds provide a suitable substrate for the attachment and growth of primary osteoblasts, and might potentially be used as bone defect fillers and in the design of tissue-engineered bone substitutes.
AB - Critical-size bone defects usually require the use of autogenous bone grafts to heal. Harvesting of bone is traumatic, and results in high morbidity of donor site. A potential alternative to bone graft may be a bone substitute with adequate biocompatibility and biological properties produced from ceramics and/or bioresorbable or biodegradable polymers. In the present study, sheep osteoblasts isolated from cancellous bone chips were seeded and cultured for 1, 2, and 3 weeks on porous poly(L/DL-lactide) 80/20% scaffolds. The cell morphology was assessed from rhodamine staining and scanning electron microscopy, cell growth, and activity from the measurements of DNA, alkaline phosphatase activity, and total protein amount in the cell lysate, and mineral deposits from EDAX and van Kossa staining. The cells attached firmly to the scaffold walls, grew deeply into the pores, and exhibited morphology typical of osteoblasts. Mineralized noduli with a Ca/P ratio of 1.4 to 1.8 found in the scaffold indicated that the cells had maintained their osteoblastic phenotype. The amount of DNA, alkaline phosphatase activity, and the total amount of proteins increased with time of culturing, although at 3 weeks the cells did not yet reach the contact inhibition. These results demonstrate that three-dimensional porous poly(L/DL-lactide) 80/20% scaffolds provide a suitable substrate for the attachment and growth of primary osteoblasts, and might potentially be used as bone defect fillers and in the design of tissue-engineered bone substitutes.
KW - Bioresorbable scaffold
KW - Bone substitute
KW - Cell culture
KW - Osteoblasts
KW - Polylactide
UR - http://www.scopus.com/inward/record.url?scp=33644843422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644843422&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.30479
DO - 10.1002/jbm.a.30479
M3 - Article
VL - 75
SP - 702
EP - 709
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 3
ER -