The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis

Bruno Travi, Y. Osorio, N. G. Saravia

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The influence of nonspecific and immunologically elicited inflammatory responses on the development of metastatic lesions was examined in the hamster model of Leishmania (Viannia) panamensis infection. Delayed type hypersensitivity (DTH) responses were induced using the contact sensitizing agent DNFB (2, 4-dinitro-1-fluorobenzene) and infection with L. panamensis followed by intradermal application of leishmanin. Nonspecific inflammatory response was achieved by the surgical excision of toes. The inductive and eliciting procedures were performed on the ears and fore and hind paws of the right side of experimental groups of hamsters that were inoculated in the snout with a highly metastatic strain of L. panamensis (MHOM/COL/84/1099). Skin metastases were detected by physical evaluation at 15-day intervals over a period of 7-8 mo. Suspected metastases were parasitologically confirmed by culture of tissue fluid aspirated from the lesion. The frequency of metastatic lesions was greater in ha rosters subjected to inflammatory stimuli (14/38) than control animals (6/33; P = 0.035). Likewise, the frequency of metastases at the site of induction and elicitation of inflammation (18/22 lesions) in the experimental groups was greater than that observed at the same site in control animals (5/11 lesions; P = 0.017). These findings support a causal relationship between inflammatory response and the development of lesions in this model of secondary disease caused by L. panamensis.

Original languageEnglish (US)
Pages (from-to)454-457
Number of pages4
JournalJournal of Parasitology
Volume82
Issue number3
DOIs
StatePublished - Jun 1996
Externally publishedYes

Fingerprint

Leishmania guyanensis
Leishmania
hamsters
lesion
lesions (animal)
metastasis
Cricetinae
inflammation
Neoplasm Metastasis
Skin
Fluorobenzenes
Haptens
Delayed Hypersensitivity
Toes
Infection
Ear
Inflammation
induced response
delayed hypersensitivity
excision

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis. / Travi, Bruno; Osorio, Y.; Saravia, N. G.

In: Journal of Parasitology, Vol. 82, No. 3, 06.1996, p. 454-457.

Research output: Contribution to journalArticle

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