The influence of dietary protein and carbohydrate on the principal oxidative biotransformations of estradiol in normal subjects

Karl Anderson, A. Kappas, A. H. Conney, H. L. Bradlow, J. Fishman

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96 Citations (Scopus)

Abstract

Dietary protein, when substituted for carbohydrate or fat, can increase cytochrome P-450-dependent drug oxidation rates in humans. Endogenous estrogens, as well as drugs, are also metabolized by cytochrome P-450 and other enzymes in the hepatic endoplasmic reticulum. Therefore, it was of interest to determine whether variations in diet can alter the major metabolic pathways for estrogens, as assessed by radiometric methods. Eight normal men were fed a high protein diet (44% of calories as protein, and 35% as carbohydrate for 2 weeks), followed by a high carbohydrate diet (70% of calories as carbohydrate and 10% as protein) for an additional 2 weeks. The fat and total energy contents of the two diets were equal. The percent oxidation of [2-3H]estradiol, measured as 3H2O released, which is an in vivo measure of 2-hydroxylation of endogenous estrogen, was greater in all 8 men during the high protein dietary period than during the high carbohydrate dietary period (44 ± 3% and 33 ± 3%, respectively, means ± SE, P < 0.005). In contrast, 16α-hydroxylation of estrogen, as measured using [16α-3H]estradiol, did not change significantly. Our findings demonstrate that dietary components can alter estradiol oxidation in humans and that the 2- and 16α-hydroxylases for estrogen are under separate regulatory control. The influences of specific nutrients on estrogen metabolism may have potential significance for diseases in which these hormones may play a role in clinical expression.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume59
Issue number1
StatePublished - 1984
Externally publishedYes

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Dietary Carbohydrates
Dietary Proteins
Biotransformation
Estradiol
Estrogens
Nutrition
Carbohydrates
Diet
Hydroxylation
Cytochrome P-450 Enzyme System
Oxidation
Period Circadian Proteins
Fats
Proteins
Metabolic Networks and Pathways
Endoplasmic Reticulum
Pharmaceutical Preparations
Mixed Function Oxygenases
Metabolism
Nutrients

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

The influence of dietary protein and carbohydrate on the principal oxidative biotransformations of estradiol in normal subjects. / Anderson, Karl; Kappas, A.; Conney, A. H.; Bradlow, H. L.; Fishman, J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 59, No. 1, 1984, p. 103-107.

Research output: Contribution to journalArticle

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