The influence of nerve growth factor on the in vitro proliferative response of rat spleen lymphocytes

L. W. Thorpe, J. R. Perez-Polo

    Research output: Contribution to journalArticle

    137 Citations (Scopus)

    Abstract

    Splenic lymphocytes and accessory cells express receptors for nerve growth factor (NGF), a well-characterized neurotropic peptide that influences the development and survival of neuronal elements in the central and peripheral nervous systems. In the present study, we report that when rat splenic mononuclear cells (MC) are incubated in the presence of NGF, a dose-dependent increase in DNA synthesis occurs during 96-120 hours of culture as measured by 3H thymidine (3H-Thd) uptake. The minimal molar concentration of NGF at which the increased proliferative response of the cells (3.7 nM) is seen corresponded to the equilibrium disassociation binding constant of the MC (K(d) = 2.5 nM), suggesting that the response was a consequence of receptor-ligand interaction. In addition, NGF was able to potentiate the lymphoproliferative response to several T-cell and B-cell mitogens. Significantly increased 3H-Thd uptake by NGF-stimulated cells was noted for concanavalin A (Con A), phytohemagglutinin (PHA), and lipopolysaccharide (LPS), particularly at suboptimal dosages of mitogen. Thus it appears that the NGF receptors on rat splenic MC are physiologically relevant and that NGF can modulate proliferation of T- and B- cells.

    Original languageEnglish (US)
    Pages (from-to)134-139
    Number of pages6
    JournalJournal of Neuroscience Research
    Volume18
    Issue number1
    StatePublished - 1987

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    Nerve Growth Factor
    Spleen
    Lymphocytes
    Nerve Growth Factor Receptors
    Mitogens
    Thymidine
    B-Lymphocytes
    Peripheral Nervous System
    Phytohemagglutinins
    Concanavalin A
    Lipopolysaccharides
    In Vitro Techniques
    Central Nervous System
    Ligands
    T-Lymphocytes
    Peptides
    DNA

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    The influence of nerve growth factor on the in vitro proliferative response of rat spleen lymphocytes. / Thorpe, L. W.; Perez-Polo, J. R.

    In: Journal of Neuroscience Research, Vol. 18, No. 1, 1987, p. 134-139.

    Research output: Contribution to journalArticle

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