The interaction of tubulin and other proteins with structure-stabilizing solvents

Serge N. Timasheff, James Lee, Eugene P. Pittz, Neil Tweedy

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A study has been carried out of the interactions with proteins of solvent systems which are commonly used to stabilize proteins in solution or to crystallize proteins out of solution. The first class consisted of water-sucrose and water-glycerol, which are frequently added to enzyme solutions in order to maintain their activity; the second class contained sodium and ammonium sulfates and 2-methyl-2,4-pentanediol (MPD) as solvent components. Densimetry and differential refractometry experiments with the application of multicomponent thermodynamic theory, showed that, in all four systems, proteins are preferentially hydrated, i.e., addition of these cosolvents results in an unfavorable free energy change. Detailed analysis of the thermodynamics of protein transitions in the presence of sucrose showed that its stabilizing action can be accounted for to a great extent in terms of the surface energy of this solvent system.

Original languageEnglish (US)
Pages (from-to)658-663
Number of pages6
JournalJournal of Colloid and Interface Science
Volume55
Issue number3
DOIs
StatePublished - 1976
Externally publishedYes

Fingerprint

Tubulin
proteins
Proteins
sucrose
Sugar (sucrose)
interactions
Sucrose
Thermodynamics
thermodynamics
ammonium sulfates
Water
Ammonium Sulfate
glycerols
sodium sulfates
Glycerol
Interfacial energy
water
Free energy
surface energy
enzymes

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

The interaction of tubulin and other proteins with structure-stabilizing solvents. / Timasheff, Serge N.; Lee, James; Pittz, Eugene P.; Tweedy, Neil.

In: Journal of Colloid and Interface Science, Vol. 55, No. 3, 1976, p. 658-663.

Research output: Contribution to journalArticle

Timasheff, Serge N. ; Lee, James ; Pittz, Eugene P. ; Tweedy, Neil. / The interaction of tubulin and other proteins with structure-stabilizing solvents. In: Journal of Colloid and Interface Science. 1976 ; Vol. 55, No. 3. pp. 658-663.
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