The interlaboratory testing of the test-system for detection of the Lumpy skin disease virus DNA using real-time PCR ‘Bovi-DNA-test-LSD virus’

Lumpy skin disease (LSD, nodular dermatitis of cattle)—is the contagious poxviral disease of cattle. It characterized with severe losses and different ranges of mortality and morbidity. The disease is endemic in many Asian and African countries.
National Scientific Center ‘Institute for Experimental and Clinical Veterinary Medicine’ in collaboration with State Scientific and Research Institute for Laboratory Diagnostics and Veterinary-Sanitary Expertise developed the in-house PCR-based protocol for LSDV detection that requires fast implementation. The evaluation of diagnostics kit has been performed under OIE requirements with determination of the sensitivity, specificity, repeatability and domain-specificity. Detection kit is recommended for practical application.