TY - JOUR
T1 - The isolation and characterization of estrogen binding proteins in the pancreas of male and female hamsters
AU - Saydjari, Rami
AU - Singh, Pomila
AU - Affini, Bashiru
AU - Townsend, Courtney M.
AU - Thompson, James C.
PY - 1988/1
Y1 - 1988/1
N2 - Specific estradiol binding proteins (EBP) that have been described in the pancreatic tissues of a number of species are thought to be important in maintaining the structural and functional integrity of the pancreas. However, possible sex-related differences in the presence and characteristics of EBP have not been examined. In the present study, we have analyzed the pancreatic tissues of male and female Syrian golden hamsters for the presence of EBP and progesterone binding protein (PBP), and further characterized these sites. Our results indicate the presence of only one class of EBP with a high capacity ( > 500 fmol/mg protein) and low affinity (Kd > 1.0 nM) in the pancreatic cytosol of female hamsters. On the other hand, there appeared to be two distinct classes of EBP in the male pancreas. One class of EBP in the male pancreas had a high binding affinity (Kd = < 0.05 nM) and low capacity (< 10 fmol/mg protein); we have arbitrarily called these Type I EBP. The second class of EBP in the male pancreas which resembled EBP in the female pancreas had a high capacity ( > 100 fmol/mg protein) and a low binding affinity (K = > 1.0 nM); we have called these Type II EBP. The sucrose-density gradient profile of EBP for male and female hamster pancreas demonstrated the presence of both an 8S binding protein and a 4S binding protein in the male pancreas; the female pancreas had only a 4S binding protein. PBP were not detected in pancreas of either male or female hamsters. We conclude that significant sex-related differences are present in the EBP populations of the hamster pancreas.
AB - Specific estradiol binding proteins (EBP) that have been described in the pancreatic tissues of a number of species are thought to be important in maintaining the structural and functional integrity of the pancreas. However, possible sex-related differences in the presence and characteristics of EBP have not been examined. In the present study, we have analyzed the pancreatic tissues of male and female Syrian golden hamsters for the presence of EBP and progesterone binding protein (PBP), and further characterized these sites. Our results indicate the presence of only one class of EBP with a high capacity ( > 500 fmol/mg protein) and low affinity (Kd > 1.0 nM) in the pancreatic cytosol of female hamsters. On the other hand, there appeared to be two distinct classes of EBP in the male pancreas. One class of EBP in the male pancreas had a high binding affinity (Kd = < 0.05 nM) and low capacity (< 10 fmol/mg protein); we have arbitrarily called these Type I EBP. The second class of EBP in the male pancreas which resembled EBP in the female pancreas had a high capacity ( > 100 fmol/mg protein) and a low binding affinity (K = > 1.0 nM); we have called these Type II EBP. The sucrose-density gradient profile of EBP for male and female hamster pancreas demonstrated the presence of both an 8S binding protein and a 4S binding protein in the male pancreas; the female pancreas had only a 4S binding protein. PBP were not detected in pancreas of either male or female hamsters. We conclude that significant sex-related differences are present in the EBP populations of the hamster pancreas.
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U2 - 10.1016/0022-4731(88)90374-3
DO - 10.1016/0022-4731(88)90374-3
M3 - Article
C2 - 3347049
AN - SCOPUS:0023831264
SN - 0022-4731
VL - 29
SP - 41
EP - 45
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 1
ER -