The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography

Thomas J. Smith

doi:10.1016/0166-0934(87)90011-5

The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography

Thomas J. Smith

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids.

Original language	English (US)
Pages (from-to)	263-269
Number of pages	7
Journal	Journal of Virological Methods
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0166-0934(87)90011-5
State	Published - Jul 1987
Externally published	Yes

Keywords

Chromatography
Cowpea mosaic virus
Purification

ASJC Scopus subject areas

Virology

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10.1016/0166-0934(87)90011-5

Cite this

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title = "The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography",

abstract = "This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids.",

keywords = "Chromatography, Cowpea mosaic virus, Purification",

author = "Smith, \{Thomas J.\}",

note = "Funding Information: A sinceret hanksgoes to Yunge Li who made theses tudiesp ossibleb y supplying large amountso f purified virus. I would also like to thank Dr John E. Johnson for stimulatingd iscussions,D r Michael G. Rossmann,w hose equipmentm ade it all possible, and Sharon Fateley for preparationo f the manuscript.T his work was supportedb y a grant from The Jane Coffins Childs Memorial Fund for Medical Research. and by a National Institutes of Health grant to Dr Michael G. Ross-mann.",

year = "1987",

month = jul,

doi = "10.1016/0166-0934(87)90011-5",

language = "English (US)",

volume = "16",

pages = "263--269",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier B.V.",

number = "4",

}

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T1 - The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography

AU - Smith, Thomas J.

N1 - Funding Information: A sinceret hanksgoes to Yunge Li who made theses tudiesp ossibleb y supplying large amountso f purified virus. I would also like to thank Dr John E. Johnson for stimulatingd iscussions,D r Michael G. Rossmann,w hose equipmentm ade it all possible, and Sharon Fateley for preparationo f the manuscript.T his work was supportedb y a grant from The Jane Coffins Childs Memorial Fund for Medical Research. and by a National Institutes of Health grant to Dr Michael G. Ross-mann.

PY - 1987/7

Y1 - 1987/7

N2 - This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids.

AB - This report describes the first time entire viral capsids have been purified using fast protein liquid chromatography (FPLC) techniques. The FPLC is used here to separate the two electrophoretic forms of cowpea mosaic virus. The capsid forms are shown to be separated by the Mono-Q column without damaging the capsids.

KW - Chromatography

KW - Cowpea mosaic virus

KW - Purification

UR - https://www.scopus.com/pages/publications/0023231573

UR - https://www.scopus.com/pages/publications/0023231573#tab=citedBy

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DO - 10.1016/0166-0934(87)90011-5

M3 - Article

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AN - SCOPUS:0023231573

SN - 0166-0934

VL - 16

SP - 263

EP - 269

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 4

ER -

The isolation of the two electrophoretic forms of cowpea mosaic virus using fast protein liquid chromatography

Abstract

Keywords

ASJC Scopus subject areas

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