The kinase defective EPHB6 receptor tyrosine kinase activates MAP kinase signaling in lung adenocarcinoma

Jun Yu, Etmar Bulk, Ping Ji, Antje Hascher, Steffen Koschmieder, Wolfgang E. Berdel, Carsten Müller-Tidow

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Decreased expression levels of EPHB6, a member of the receptor tyrosine kinases (RTKs), are associated with an increased risk of metastasis development in early stage non-small cell lung cancer (NSCLC). However, the signaling properties of the kinase-defective EPHB6 receptor are not well-understood. Here, we show that expression of EPHB6 in A549 lung adenocarinoma cells led to phosphorylation of the MAP kinase ERK. Conversely, siRNA based knockdown of EPHB6 reversed ERK phosphorylation. Intriguingly, EPHB6-induced phosphorylation of ERK was uncoupled by activation of the Elk-1 transcriptional factor. These analyses suggest that kinase defective EPHB6 can lead to MAPK activation.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalInternational Journal of Oncology
Volume35
Issue number1
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Mitogen-Activated Protein Kinase Kinases
Protein-Tyrosine Kinases
Phosphotransferases
Phosphorylation
Receptor Protein-Tyrosine Kinases
Non-Small Cell Lung Carcinoma
Small Interfering RNA
Neoplasm Metastasis
Lung
Adenocarcinoma of lung

Keywords

  • Elk-1
  • EPHB6
  • Extracellular-signal-regulated kinase
  • Mitogen-activated protein kinase
  • Non-small cell lung cancer
  • Receptor tyrosine kinase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The kinase defective EPHB6 receptor tyrosine kinase activates MAP kinase signaling in lung adenocarcinoma. / Yu, Jun; Bulk, Etmar; Ji, Ping; Hascher, Antje; Koschmieder, Steffen; Berdel, Wolfgang E.; Müller-Tidow, Carsten.

In: International Journal of Oncology, Vol. 35, No. 1, 2009, p. 175-179.

Research output: Contribution to journalArticle

Yu, Jun ; Bulk, Etmar ; Ji, Ping ; Hascher, Antje ; Koschmieder, Steffen ; Berdel, Wolfgang E. ; Müller-Tidow, Carsten. / The kinase defective EPHB6 receptor tyrosine kinase activates MAP kinase signaling in lung adenocarcinoma. In: International Journal of Oncology. 2009 ; Vol. 35, No. 1. pp. 175-179.
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