The leading edge is the primary force producing component of migrating fibroblasts

Adam S. Meshel, Michael Sheetz

Research output: Contribution to journalConference article

Abstract

To examine how cells generate force at the level of single extracellular matrix fibers, a miniature force-transducer system was created around a novel microfabricated silicon device. We characterize the development of isometric force on single collagen type-I fibers. We show that cells remodel individual collagen fibers by moving them inward using a hand-over-hand cycle of binding, movement, and release. This remodelling is caused by only a 2-3 micrometer region of active lamellapodium. We also show that movement of individual fibers is rapid at low restrictive forces, and this velocity quickly decreases as forces approach 60 pN. A single cell is capable of generating between 180-250 pN of force on a single fiber. This data supports the hypothesis that the leading edge of migrating cells is primarily responsible for generating force on the substrate.

Original languageEnglish (US)
Pages (from-to)296-297
Number of pages2
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Fingerprint

Fibroblasts
Fibers
Collagen
Hand
Silicon
Collagen Type I
Transducers
Extracellular Matrix
Equipment and Supplies
Substrates

Keywords

  • Biomechanics
  • Cell adhesion
  • Cell movement
  • Extracellular matrix/collagen
  • Force measurements
  • Myosin II
  • Nanofabrication

ASJC Scopus subject areas

  • Bioengineering

Cite this

@article{c78643e0f81249efaa3ea28b8e7a835b,
title = "The leading edge is the primary force producing component of migrating fibroblasts",
abstract = "To examine how cells generate force at the level of single extracellular matrix fibers, a miniature force-transducer system was created around a novel microfabricated silicon device. We characterize the development of isometric force on single collagen type-I fibers. We show that cells remodel individual collagen fibers by moving them inward using a hand-over-hand cycle of binding, movement, and release. This remodelling is caused by only a 2-3 micrometer region of active lamellapodium. We also show that movement of individual fibers is rapid at low restrictive forces, and this velocity quickly decreases as forces approach 60 pN. A single cell is capable of generating between 180-250 pN of force on a single fiber. This data supports the hypothesis that the leading edge of migrating cells is primarily responsible for generating force on the substrate.",
keywords = "Biomechanics, Cell adhesion, Cell movement, Extracellular matrix/collagen, Force measurements, Myosin II, Nanofabrication",
author = "Meshel, {Adam S.} and Michael Sheetz",
year = "2002",
month = "12",
day = "1",
language = "English (US)",
volume = "1",
pages = "296--297",
journal = "Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference",
issn = "1557-170X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

TY - JOUR

T1 - The leading edge is the primary force producing component of migrating fibroblasts

AU - Meshel, Adam S.

AU - Sheetz, Michael

PY - 2002/12/1

Y1 - 2002/12/1

N2 - To examine how cells generate force at the level of single extracellular matrix fibers, a miniature force-transducer system was created around a novel microfabricated silicon device. We characterize the development of isometric force on single collagen type-I fibers. We show that cells remodel individual collagen fibers by moving them inward using a hand-over-hand cycle of binding, movement, and release. This remodelling is caused by only a 2-3 micrometer region of active lamellapodium. We also show that movement of individual fibers is rapid at low restrictive forces, and this velocity quickly decreases as forces approach 60 pN. A single cell is capable of generating between 180-250 pN of force on a single fiber. This data supports the hypothesis that the leading edge of migrating cells is primarily responsible for generating force on the substrate.

AB - To examine how cells generate force at the level of single extracellular matrix fibers, a miniature force-transducer system was created around a novel microfabricated silicon device. We characterize the development of isometric force on single collagen type-I fibers. We show that cells remodel individual collagen fibers by moving them inward using a hand-over-hand cycle of binding, movement, and release. This remodelling is caused by only a 2-3 micrometer region of active lamellapodium. We also show that movement of individual fibers is rapid at low restrictive forces, and this velocity quickly decreases as forces approach 60 pN. A single cell is capable of generating between 180-250 pN of force on a single fiber. This data supports the hypothesis that the leading edge of migrating cells is primarily responsible for generating force on the substrate.

KW - Biomechanics

KW - Cell adhesion

KW - Cell movement

KW - Extracellular matrix/collagen

KW - Force measurements

KW - Myosin II

KW - Nanofabrication

UR - http://www.scopus.com/inward/record.url?scp=0036907729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036907729&partnerID=8YFLogxK

M3 - Conference article

VL - 1

SP - 296

EP - 297

JO - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference

JF - Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference

SN - 1557-170X

ER -