The linker region plays an important role in the interdomain communication of the response regulator OmpR

Kirsten Mattison, Ricardo Oropeza, Linda Kenney

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

OmpR is the response regulator of a two-component regulatory system that controls the expression of the porin genes ompF and ompC in Escherichia coli. This regulator consists of two domains joined by a flexible linker region. The amino-terminal domain is phosphorylated by the sensor kinase EnvZ, and the carboxyl- terminal domain binds DNA via a winged helix-turn-helix motif. In vitro studies have shown that amino-terminal phosphorylation enhances the DNA binding affinity of OmpR and, conversely, that DNA binding by the carboxyl terminus increases OmpR phosphorylation. In the present work, we demonstrate that the linker region contributes to this communication between the two domains of OmpR. Changing the specific amino acid composition of the linker alters OmpR function, as does increasing or decreasing its length. Three linker mutants give rise to an OmpF+ OmpC- phenotype, but the defects are not due to a shared molecular mechanism. Currently, functional homology between response regulators is predicted based on similarities in the amino and carboxyl-terminal domains. The results presented here indicate that linker length and composition should also be considered. Furthermore, classification of response regulators in the same subfamily does not necessarily imply that they share a common response mechanism.

Original languageEnglish (US)
Pages (from-to)32714-32721
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number36
DOIs
StatePublished - Sep 6 2002
Externally publishedYes

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Phosphorylation
DNA
Communication
Helix-Turn-Helix Motifs
Porins
Chemical analysis
Escherichia coli
Phosphotransferases
Genes
Phenotype
Control systems
Gene Expression
Amino Acids
Defects
Sensors
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

The linker region plays an important role in the interdomain communication of the response regulator OmpR. / Mattison, Kirsten; Oropeza, Ricardo; Kenney, Linda.

In: Journal of Biological Chemistry, Vol. 277, No. 36, 06.09.2002, p. 32714-32721.

Research output: Contribution to journalArticle

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