The location of San Angelo virus in developing ovaries of transovarially infected Aedes albopictus mosquitoes as revealed by fluorescent antibody technique

R. B. Tesh, M. Cornet

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aedes albopictus adult female mosquitoes, transovarially infected with San Angelo (SA) virus, were examined by fluorescent antibody technique during various stages of ovarian development to determine how the virus enters the egg. Upon emergence from the pupal stage, viral antigen was observed only in the oviduct and ovariole sheath. By the 4th day of adulthood, it was visible in the follicular epithelium, oocytes and nurse cells of the primary follicles. In the 72-hour period between the ingestion of blood and oviposition, there was a marked increase in the amount of viral antigen in the oocyte, indicating rapid virus accumulation. After oviposition, SA viral antigen was also seen in the secondary ovarian follicles. The observed sequence of infection of the mosquito ovariole with SA virus is analogous to that described with certain endosymbionts of insects.

Original languageEnglish (US)
Pages (from-to)212-218
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume30
Issue number1
StatePublished - 1981
Externally publishedYes

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Aedes
Culicidae
Fluorescent Antibody Technique
Viral Antigens
Ovary
Viruses
Oviposition
Oocytes
Ovarian Follicle
Oviducts
Ovum
Insects
Epithelium
Eating
Nurses
Infection

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

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abstract = "Aedes albopictus adult female mosquitoes, transovarially infected with San Angelo (SA) virus, were examined by fluorescent antibody technique during various stages of ovarian development to determine how the virus enters the egg. Upon emergence from the pupal stage, viral antigen was observed only in the oviduct and ovariole sheath. By the 4th day of adulthood, it was visible in the follicular epithelium, oocytes and nurse cells of the primary follicles. In the 72-hour period between the ingestion of blood and oviposition, there was a marked increase in the amount of viral antigen in the oocyte, indicating rapid virus accumulation. After oviposition, SA viral antigen was also seen in the secondary ovarian follicles. The observed sequence of infection of the mosquito ovariole with SA virus is analogous to that described with certain endosymbionts of insects.",
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