The long polar fimbriae of STEC O157

H7 induce expression of pro-inflammatory markers by intestinal epithelial cells

Mauricio J. Farfan, Lidia Cantero, Alejandra Vergara, Roberto Vidal, Alfredo Torres

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Infection with Shiga toxin-producing Escherichia coli (STEC) O157:H7 is characterized by acute inflammation of the colonic mucosa. STEC O157:H7 contains two non-identical loci encoding long polar fimbriae (Lpf), which play a role in the STEC colonization of the intestinal epithelial cells. However, no information is available regarding the involvement of Lpf in the STEC-induced host inflammatory response. Hence, in this study we assess the role of Lpf as an inducer of inflammation on intestinal epithelial cells. Secretion of pro-inflammatory cytokines in response to STEC wild type and lpf isogenic mutants was evaluated on intestinal T84 cells. Of the 27 cytokines assayed, IL-6, IL-8, IL-15, FGF, GM-CSF and IP-10 were significantly reduced, when compared to the wild-type strain, in the lpfA1 lpfA2 double mutant. Further, the host intracellular signaling pathways activated in response to Lpf were determined by using an array containing genes representative of 18 different signal transduction pathways. The analysis indicated that the NF-κB pathway is activated in response to Lpf-expressing STEC. Therefore, our study supports the role of Lpf as a STEC factor mediating intestinal inflammation.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume152
Issue number1-2
DOIs
StatePublished - Mar 15 2013

Fingerprint

Shiga-Toxigenic Escherichia coli
Escherichia coli O157
fimbriae
Shiga toxin-producing Escherichia coli
epithelial cells
Epithelial Cells
inflammation
Inflammation
cytokines
mutants
Cytokines
interleukin-8
Interleukin-15
interleukin-6
mucosa
Granulocyte-Macrophage Colony-Stimulating Factor
signal transduction
Interleukin-8
Interleukin-6
Signal Transduction

Keywords

  • Enterohemorrhagic E. coli
  • Inflammation
  • Interleukin-8
  • Long polar fimbriae
  • Shiga toxin E. coli

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

The long polar fimbriae of STEC O157 : H7 induce expression of pro-inflammatory markers by intestinal epithelial cells. / Farfan, Mauricio J.; Cantero, Lidia; Vergara, Alejandra; Vidal, Roberto; Torres, Alfredo.

In: Veterinary Immunology and Immunopathology, Vol. 152, No. 1-2, 15.03.2013, p. 126-131.

Research output: Contribution to journalArticle

Farfan, Mauricio J. ; Cantero, Lidia ; Vergara, Alejandra ; Vidal, Roberto ; Torres, Alfredo. / The long polar fimbriae of STEC O157 : H7 induce expression of pro-inflammatory markers by intestinal epithelial cells. In: Veterinary Immunology and Immunopathology. 2013 ; Vol. 152, No. 1-2. pp. 126-131.
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