The long polar fimbriae operon and its flanking regions in bovine Escherichia coli O157: H43 and STEC O136: H12 strains

Domonkos Sváb, Lucia Galli, Balázs Horváth, Gergely Maróti, Ulrich Dobrindt, Alfredo Torres, Marta Rivas, István Tóth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative atypical E. coli O157:H43 strain of bovine origin and also sequenced the lpf2-1 operon of six additional atypical O157 bovine E. coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 E. coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain, and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci are highly conserved in E. coli isolates; however, its role in adherence might be masked by other uncharacterized adhesins. This paper highlights the diversity of the Escherichia coli species and one of the virulence factors of these pathogens of animals and man.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalPathogens and Disease
Volume68
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Shiga-Toxigenic Escherichia coli
Escherichia coli O157
Operon
Escherichia coli
Virulence Factors
Alleles
Shiga Toxin
Nucleotides

Keywords

  • Atypical E. coli
  • Escherichia coli
  • Long polar fimbriae
  • O136:H12
  • O157:H43
  • Pathogenic E. coli

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

The long polar fimbriae operon and its flanking regions in bovine Escherichia coli O157 : H43 and STEC O136: H12 strains. / Sváb, Domonkos; Galli, Lucia; Horváth, Balázs; Maróti, Gergely; Dobrindt, Ulrich; Torres, Alfredo; Rivas, Marta; Tóth, István.

In: Pathogens and Disease, Vol. 68, No. 1, 2013, p. 1-7.

Research output: Contribution to journalArticle

Sváb, Domonkos ; Galli, Lucia ; Horváth, Balázs ; Maróti, Gergely ; Dobrindt, Ulrich ; Torres, Alfredo ; Rivas, Marta ; Tóth, István. / The long polar fimbriae operon and its flanking regions in bovine Escherichia coli O157 : H43 and STEC O136: H12 strains. In: Pathogens and Disease. 2013 ; Vol. 68, No. 1. pp. 1-7.
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