The lpf gene cluster for long polar fimbriae is not involved in adherence of enteropathogenic Escherichia coli or virulence of Citrobacter rodentium

Ichiro Tatsuno, Rosanna Mundy, Gad Frankel, Yuwen Chong, Alan D. Phillips, Alfredo Torres, James B. Kaper

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Using the enteropathogenic Escherichia coli (EPEC) genome sequence, we found that EPEC E2348/69 has an lpfABCDE gene cluster homologous (about 60% identical at the protein level) to the Salmonella long polar fimbria (LPF) operon. To determine whether this operon is essential for adherence, the lpfABCDE23 genes were deleted from EPEC strain E2348/69 by allelic exchange. Analysis of the resulting EPECΔlpfABCDE23 strain showed no change in adherence to HeLa cells or to human intestinal biopsy cells in the in vitro organ culture (FVOC) system compared to the wild type. Sera from volunteers experimentally infected with E2348/69 showed no antibody response to the major subunit protein, LpfA. These results suggested that the lpf E23 gene cluster is not necessary for EPEC adherence and attaching/effacing (A/E) lesion formation on human biopsy samples and is not expressed during human infection. We also identified an lpf gene cluster in Citrobacter rodentium strain ICC168 (lpfcr). A ΔlpfA cr mutant of ICC168 retained wild-type adherence and A/E lesion-forming activity on HeLa cells. C3H/HeJ mice were infected with a wild-type C. rodentium strain and its lpfAcr isogenic mutant. Both strains were recovered at high levels in stools, and there were no significant differences between the groups both in terms of the number of CFU/organ (colon and cecum) and in terms of the amount of hyperplasia, as measured by weight. Similar results were observed in a second mouse strain, C57BL/6. These data suggest that in addition to playing no apparent role in EPEC pathogenesis, lpfcr is not required for C. rodentium virulence in either the C3H/HeJ or C57BL/6 mouse model.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalInfection and Immunity
Volume74
Issue number1
DOIs
StatePublished - Jan 2006

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Citrobacter rodentium
Enteropathogenic Escherichia coli
Multigene Family
Virulence
Operon
Inbred C57BL Mouse
HeLa Cells
Biopsy
Cecum
Inbred C3H Mouse
Organ Culture Techniques
Protein Subunits
Salmonella
Antibody Formation
Hyperplasia
Volunteers
Colon
Genome
Weights and Measures
Infection

ASJC Scopus subject areas

  • Immunology

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The lpf gene cluster for long polar fimbriae is not involved in adherence of enteropathogenic Escherichia coli or virulence of Citrobacter rodentium. / Tatsuno, Ichiro; Mundy, Rosanna; Frankel, Gad; Chong, Yuwen; Phillips, Alan D.; Torres, Alfredo; Kaper, James B.

In: Infection and Immunity, Vol. 74, No. 1, 01.2006, p. 265-272.

Research output: Contribution to journalArticle

Tatsuno, Ichiro ; Mundy, Rosanna ; Frankel, Gad ; Chong, Yuwen ; Phillips, Alan D. ; Torres, Alfredo ; Kaper, James B. / The lpf gene cluster for long polar fimbriae is not involved in adherence of enteropathogenic Escherichia coli or virulence of Citrobacter rodentium. In: Infection and Immunity. 2006 ; Vol. 74, No. 1. pp. 265-272.
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