The Makona Variant of Ebola Virus Is Highly Lethal to Immunocompromised Mice and Immunocompetent Ferrets

Gary Wong, Anders Leung, Shihua He, Wenguang Cao, Marc Antoine De La Vega, Bryan D. Griffin, Geoff Soule, Gary P. Kobinger, Darwyn Kobasa, Xiangguo Qiu

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

During 2013-2016, a novel isolate of Ebola virus (EBOV-Makona) caused an epidemic in West Africa. The virus was distinct from known EBOV strains (EBOV-Kikwit and EBOV-Mayinga), which were responsible for previous outbreaks in Central Africa. To investigate the pathogenicity of EBOV-Makona, we engineered and rescued an early isolate (H.sapiens-wt/GIN/2014/Makona- Gueckedou-C07, called rgEBOV-C07) using an updated reverse-genetics system. rgEBOV-C07 was found to be highly pathogenic in both the knockout mouse and ferret models, with median lethal dose values of 0.078 and 0.015 plaque-forming units, respectively. Therefore, these animals are appropriate for screening potential countermeasures against EBOV-Makona without the need for species adaptation.

Original languageEnglish (US)
Pages (from-to)S466-S470
JournalJournal of Infectious Diseases
Volume218
DOIs
StatePublished - Nov 22 2018
Externally publishedYes

Keywords

  • Ebola
  • ferrets
  • knockout mice
  • pathogenicity
  • reverse genetics.

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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