The measurement of the isoflavone daidzein by time resolved fluorescent immunoassay: A method for assessment of dietary soya exposure

F. Kohen, S. Lichter, B. Gayer, J. Deboever, L. J.W. Lu

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

We report a novel method for the measurement of urinary daidzein that is suitable for assessment of dietary soya exposure. The method incorporates the following features: (i) a highly specific monoclonal antibody to daidzein (clone 4E4) raised through the 7 position of daidzein and (ii) a europium labeled ovalbumin daidzein conjugate. In the present format, dilute urine samples of subjects who ingested soy milk are hydrolyzed with β-glucuronidase for 30 min on rabbit anti-mouse coated plants. Afterwards, the specific monoclonal antibody to daidzein, clone 4E4, and europium labeled ovalbumin daidzein conjugate are added. After 1 h incubation, the wall bound fluorescence of europium is measured by time resolved fluorescence and is inversely proportional to the concentration of daidzein over the range 0.1-10 ng daidzein/well. The method demonstrates good sensitivity, precision and comparability with the chemical method GC-FID. Unlike the chemical method, the present immunoassay technique for daidzein is applicable for the measurement of large amounts of samples in epidemiological studies of the assessment and monitoring of human exposure to soya food.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume64
Issue number3-4
DOIs
StatePublished - Feb 1 1998

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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