The measurement of the isoflavone daidzein by time resolved fluorescent immunoassay: A method for assessment of dietary soya exposure

F. Kohen, S. Lichter, B. Gayer, J. Deboever, Leejane Lu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We report a novel method for the measurement of urinary daidzein that is suitable for assessment of dietary soya exposure. The method incorporates the following features: (i) a highly specific monoclonal antibody to daidzein (clone 4E4) raised through the 7 position of daidzein and (ii) a europium labeled ovalbumin daidzein conjugate. In the present format, dilute urine samples of subjects who ingested soy milk are hydrolyzed with β-glucuronidase for 30 min on rabbit anti-mouse coated plants. Afterwards, the specific monoclonal antibody to daidzein, clone 4E4, and europium labeled ovalbumin daidzein conjugate are added. After 1 h incubation, the wall bound fluorescence of europium is measured by time resolved fluorescence and is inversely proportional to the concentration of daidzein over the range 0.1-10 ng daidzein/well. The method demonstrates good sensitivity, precision and comparability with the chemical method GC-FID. Unlike the chemical method, the present immunoassay technique for daidzein is applicable for the measurement of large amounts of samples in epidemiological studies of the assessment and monitoring of human exposure to soya food.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume64
Issue number3-4
DOIs
StatePublished - Feb 1 1998

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Isoflavones
Immunoassay
Europium
Ovalbumin
Clone Cells
Fluorescence
Soy Milk
Monoclonal Antibodies
Epidemiological Monitoring
daidzein
Glucuronidase
Epidemiologic Studies
Urine
Rabbits
Food
Monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

The measurement of the isoflavone daidzein by time resolved fluorescent immunoassay : A method for assessment of dietary soya exposure. / Kohen, F.; Lichter, S.; Gayer, B.; Deboever, J.; Lu, Leejane.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 64, No. 3-4, 01.02.1998, p. 217-222.

Research output: Contribution to journalArticle

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