The mechanical hierarchies of fibronectin observed with single-molecule AFM

Andres Oberhauser, Carmelu Badilla-Fernandez, Mariano Carrion-Vazquez, Julio M. Fernandez

Research output: Contribution to journalArticle

297 Citations (Scopus)

Abstract

Mechanically induced conformational changes in proteins such as fibronectin are thought to regulate the assembly of the extracellular matrix and underlie its elasticity and extensibility. Fibronectin contains a region of tandem repeats of up to 15 type III domains that play critical roles in cell binding and self-assembly. Here, we use single-molecule force spectroscopy to examine the mechanical properties of fibronectin (FN) and its individual FNIII domains. We found that fibronectin is highly extensible due to the unfolding of its FNIII domains. We found that the native FNIII region displays strong mechanical unfolding hierarchies requiring 80 pN of force to unfold the weakest domain and 200 pN for the most stable domain. In an effort to determine the identity of the weakest/strongest domain, we engineered polyproteins composed of an individual domain and measured their mechanical stability by single-protein atomic force microscopy (AFM) techniques. In contrast to chemical and thermal measurements of stability, we found that the tenth FNIII domain is mechanically the weakest and that the first and second FNIII domains are the strongest. Moreover, we found that the first FNIII domain can acquire multiple, partially folded conformations, and that their incidence is modulated strongly by its neighbor FNIII domain. The mechanical hierarchies of fibronectin demonstrated here may be important for the activation of fibrillogenesis and matrix assembly.

Original languageEnglish (US)
Pages (from-to)433-447
Number of pages15
JournalJournal of Molecular Biology
Volume319
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Atomic Force Microscopy
Fibronectins
Polyproteins
Tandem Repeat Sequences
Protein Stability
Elasticity
Extracellular Matrix
Hot Temperature
Incidence
Proteins

Keywords

  • AFM
  • Fibronectin
  • Hierarchies
  • Mechanical unfolding
  • Single molecule

ASJC Scopus subject areas

  • Virology

Cite this

The mechanical hierarchies of fibronectin observed with single-molecule AFM. / Oberhauser, Andres; Badilla-Fernandez, Carmelu; Carrion-Vazquez, Mariano; Fernandez, Julio M.

In: Journal of Molecular Biology, Vol. 319, No. 2, 2002, p. 433-447.

Research output: Contribution to journalArticle

Oberhauser, Andres ; Badilla-Fernandez, Carmelu ; Carrion-Vazquez, Mariano ; Fernandez, Julio M. / The mechanical hierarchies of fibronectin observed with single-molecule AFM. In: Journal of Molecular Biology. 2002 ; Vol. 319, No. 2. pp. 433-447.
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