The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

Sandra L. Dabora, Michael Sheetz

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalCell
Volume54
Issue number1
DOIs
StatePublished - Jul 1 1988
Externally publishedYes

Fingerprint

Cell Extracts
Microtubules
Endoplasmic Reticulum
Cultured Cells
Cells
Membranes
Interference Microscopy
Network components
Mitochondria
Golgi Apparatus
Chick Embryo
Fibroblasts
Cell membranes
Detergents
Microscopic examination
Buffers
Adenosine Triphosphate
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts. / Dabora, Sandra L.; Sheetz, Michael.

In: Cell, Vol. 54, No. 1, 01.07.1988, p. 27-35.

Research output: Contribution to journalArticle

@article{a48ff2829ae542e88d0765fc962bd746,
title = "The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts",
abstract = "The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.",
author = "Dabora, {Sandra L.} and Michael Sheetz",
year = "1988",
month = "7",
day = "1",
doi = "10.1016/0092-8674(88)90176-6",
language = "English (US)",
volume = "54",
pages = "27--35",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",

}

TY - JOUR

T1 - The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

AU - Dabora, Sandra L.

AU - Sheetz, Michael

PY - 1988/7/1

Y1 - 1988/7/1

N2 - The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

AB - The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

UR - http://www.scopus.com/inward/record.url?scp=0024041810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024041810&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(88)90176-6

DO - 10.1016/0092-8674(88)90176-6

M3 - Article

C2 - 3289756

AN - SCOPUS:0024041810

VL - 54

SP - 27

EP - 35

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1

ER -