The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

Sandra L. Dabora; Michael F. Sheetz

doi:10.1016/0092-8674(88)90176-6

The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

Sandra L. Dabora, Michael F. Sheetz

Research output: Contribution to journal › Article › peer-review

303 Scopus citations

Abstract

The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

Original language	English (US)
Pages (from-to)	27-35
Number of pages	9
Journal	Cell
Volume	54
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(88)90176-6
State	Published - Jul 1 1988
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(88)90176-6

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title = "The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts",

abstract = "The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.",

author = "Dabora, {Sandra L.} and Sheetz, {Michael F.}",

note = "Funding Information: We wish to thank the labs of Dr. M. Schlessinger and Dr. S. Schles-singer for their generous contribution of chick embryo fibroblasts, Dr. J. E. Heuser and lab for providing the deep etch electron micrographs, Dr. D. Bole for his gift of the anti-BiP antibody, Dr. R D. Stahl for use of his Zeiss standard microscope, Dr. F~ C. Bridgman for help with freeze substitution, and the members of the Sheetz lab for helpful discussions. We also thank Dr. L. Jaffe and the University of Connecticut Graduate Programs Committee for their support of this work. These studies were supported by National institutes of Health grants GM-36277 and NS-23345. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked {"}advertisement{"} in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.",

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T1 - The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

AU - Dabora, Sandra L.

AU - Sheetz, Michael F.

N1 - Funding Information: We wish to thank the labs of Dr. M. Schlessinger and Dr. S. Schles-singer for their generous contribution of chick embryo fibroblasts, Dr. J. E. Heuser and lab for providing the deep etch electron micrographs, Dr. D. Bole for his gift of the anti-BiP antibody, Dr. R D. Stahl for use of his Zeiss standard microscope, Dr. F~ C. Bridgman for help with freeze substitution, and the members of the Sheetz lab for helpful discussions. We also thank Dr. L. Jaffe and the University of Connecticut Graduate Programs Committee for their support of this work. These studies were supported by National institutes of Health grants GM-36277 and NS-23345. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

PY - 1988/7/1

Y1 - 1988/7/1

N2 - The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

AB - The formation of a dynamic tubulovesicular membrane network that resembles the endoplasmic reticulum (ER) has been observed in extracts of cultured chick embryo fibroblasts (CEF cells) using video-enhanced differential interference contrast microscopy. Initially, membranes in the CEF extracts appeared amorphous and aggregated, but with time, membrane tubules moved out along stationary microtubules. The membrane tubules formed new branches on intersecting microtubules and fused with other branches to form a network of interconnected polygons. The tubulovesicular network was solubilized by detergent and took on a beaded morphology in a hypotonic buffer. Formation of the tubulovesicular network required ATP and microtubules. The network did not contain elements of the plasma membrane, Golgi apparatus, or mitochondria but could be labeled with ER markers. We suggest that the tubulovesicular network contains components from the ER and is formed by membrane associated motors moving upon microtubules in a process we call microtubule-dependent tethering.

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The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts

Abstract

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