The Mucosal Adjuvanticity of Cholera Toxin Involves Enhancement of Costimulatory Activity by Selective Up-Regulation of B7.2 Expression

Yingzi Cong, Casey T. Weaver, Charles O. Elson

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Abstract

Cholera toxin (CT) is a potent mucosal immunogen and adjuvant that can strongly prime mucosal T cells. The present study was undertaken to investigate the effects of CT on the expression and functional activity of the costimulatory molecules B7.1 and B7.2 on macrophages and the relationship of these effects to the mucosal adjuvanticity of CT. Bone marrow macrophages (BMM) were generated by culturing bone marrow with macrophage CSF or granulocyte-macrophage CSF. After treatment with either CT alone or IFN-γ alone, B7.2 expression on BMM was moderately up-regulated and was further increased when BMM were treated with both CT and IFN-γ together. Interestingly, CT had no effect on B7.1 expression despite the close relationship between these two molecules. Up-regulation of B7.2 expression by CT was mediated by intracellular cAMP production, in that CT-B subunit had no effect and dibutyryl cAMP could mimic the effect. CT increased functional costimulatory activity of macrophages for both anti-CD3-stimulated and allostimulated T cells, an increase that was blocked by anti-B7.2, but not anti-B7.1, Ab. B7.2 expression by Mac1+ Peyer's patch cells was increased after intraluminal exposure to CT in vivo. Treatment of mice with anti-B7.2 Ab in vivo inhibited both the mucosal adjuvanticity and the immunogenicity of CT. We conclude that CT enhances the costimulatory activity of mucosal APC by differentially up-regulating B7.2 expression, an effect that appears to be important for its mucosal adjuvanticity and immunogenicity.

Original languageEnglish (US)
Pages (from-to)5301-5308
Number of pages8
JournalJournal of Immunology
Volume159
Issue number11
StatePublished - Dec 1 1997
Externally publishedYes

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Cholera Toxin
Up-Regulation
Macrophages
Bone Marrow
T-Lymphocytes
Peyer's Patches
Granulocytes

ASJC Scopus subject areas

  • Immunology

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The Mucosal Adjuvanticity of Cholera Toxin Involves Enhancement of Costimulatory Activity by Selective Up-Regulation of B7.2 Expression. / Cong, Yingzi; Weaver, Casey T.; Elson, Charles O.

In: Journal of Immunology, Vol. 159, No. 11, 01.12.1997, p. 5301-5308.

Research output: Contribution to journalArticle

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