The nature of the prostaglandin-producing mononuclear cell in human peripheral blood

A. D. Bankhurst, E. Hastain, J. S. Goodwin, G. T. Peake

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Abstract

The purpose of this investigation was to identify the human peripheral mononuclear cell responsible for PGE production. The prostaglandin-producing cell had the following characteristics: (1) was glass adherent; (2) was removed by technique that effectively removed phagocytic cells (carbonyl iron); and (3) was not an E-rosette cell. Furthermore, the peripheral mononuclear cells that contain cytoplasmic PGE by indirect immunofluorescence also ingest latex particles. B and T lymphoid cell lines did not produce large quantities of PGE. Mononuclear cells separated on discontinuous BSA gradients varied greatly in their ability to produce prostaglandin (low density>high density). These data constitute strong evidence that the majority of PGE produced in peripheral mononuclear cell suspensions emanates from a population of low-density monocytes.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume97
Issue number2
StatePublished - Jan 1 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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