The nature of the prostaglandin-producing mononuclear cell in human peripheral blood

A. D. Bankhurst, E. Hastain, James Goodwin, G. T. Peake

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The purpose of this investigation was to identify the human peripheral mononuclear cell responsible for PGE production. The prostaglandin-producing cell had the following characteristics: (1) was glass adherent; (2) was removed by technique that effectively removed phagocytic cells (carbonyl iron); and (3) was not an E-rosette cell. Furthermore, the peripheral mononuclear cells that contain cytoplasmic PGE by indirect immunofluorescence also ingest latex particles. B and T lymphoid cell lines did not produce large quantities of PGE. Mononuclear cells separated on discontinuous BSA gradients varied greatly in their ability to produce prostaglandin (low density>high density). These data constitute strong evidence that the majority of PGE produced in peripheral mononuclear cell suspensions emanates from a population of low-density monocytes.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume97
Issue number2
StatePublished - 1981
Externally publishedYes

Fingerprint

Prostaglandins E
Prostaglandins
Blood
Latex
Suspensions
Iron
Cells
Phagocytes
Indirect Fluorescent Antibody Technique
Population Density
Microspheres
Glass
Monocytes
Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The nature of the prostaglandin-producing mononuclear cell in human peripheral blood. / Bankhurst, A. D.; Hastain, E.; Goodwin, James; Peake, G. T.

In: Journal of Laboratory and Clinical Medicine, Vol. 97, No. 2, 1981, p. 179-186.

Research output: Contribution to journalArticle

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