The neuropeptidomics of Ixodes scapularis synganglion

Susanne Neupert, William Russell, Reinhard Predel, David H. Russell, Otto F. Strey, Pete D. Teel, Ronald J. Nachman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Ticks (Ixodoidea) likely transmit the greatest variety of human and animal pathogens of any arthropod vector. Despite their medical significance little data is available about the messenger molecules in the central nervous system that coordinate all physiological processes in these animals, including behaviour. In our study, we performed the first comprehensive neuropeptidomic analysis of a tick species by using MALDI-TOF mass spectrometry. Specifically we analyzed the neuropeptides in the synganglion of Ixodes scapularis. The forthcoming sequence of the genome of this species will represent the first genomic analysis of a member of the large subphylum Chelicerata. For our approach we used information from predicted neuropeptide precursor sequences found in EST databases [Christie, AE. Neuropeptide discovery in Ixodoidea: an in silico investigation using publicly accessible expressed sequence tags. Gen Comp Endocrinol 2008;157:174-185] as well as data obtained by complete de novo sequencing. The direct tissue profiling yielded 20 neuropeptides from 12 neuropeptide precursors. The sequences of these neuropeptides are not as unique as predicted; a comparison with the peptidome of other invertebrates shows a close relationship with insect neuropeptides. This work will provide a resource for studying tick neurobiology and will hopefully also help to identify novel targets for tick and tick-borne disease control.

Original languageEnglish (US)
Pages (from-to)1040-1045
Number of pages6
JournalJournal of Proteomics
Volume72
Issue number6
DOIs
StatePublished - Aug 20 2009
Externally publishedYes

Fingerprint

Ixodes
Neuropeptides
Ticks
Expressed Sequence Tags
Animals
Arthropod Vectors
Tick-Borne Diseases
Physiological Phenomena
Disease control
Animal Behavior
Neurobiology
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Neurology
Pathogens
Invertebrates
Computer Simulation
Mass spectrometry
Insects
Mass Spectrometry
Central Nervous System

Keywords

  • Acari
  • Amblyomma
  • Ixodes
  • MALDI-TOF mass spectrometry
  • Tick neuropeptidomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Neupert, S., Russell, W., Predel, R., Russell, D. H., Strey, O. F., Teel, P. D., & Nachman, R. J. (2009). The neuropeptidomics of Ixodes scapularis synganglion. Journal of Proteomics, 72(6), 1040-1045. https://doi.org/10.1016/j.jprot.2009.06.007

The neuropeptidomics of Ixodes scapularis synganglion. / Neupert, Susanne; Russell, William; Predel, Reinhard; Russell, David H.; Strey, Otto F.; Teel, Pete D.; Nachman, Ronald J.

In: Journal of Proteomics, Vol. 72, No. 6, 20.08.2009, p. 1040-1045.

Research output: Contribution to journalArticle

Neupert, S, Russell, W, Predel, R, Russell, DH, Strey, OF, Teel, PD & Nachman, RJ 2009, 'The neuropeptidomics of Ixodes scapularis synganglion', Journal of Proteomics, vol. 72, no. 6, pp. 1040-1045. https://doi.org/10.1016/j.jprot.2009.06.007
Neupert S, Russell W, Predel R, Russell DH, Strey OF, Teel PD et al. The neuropeptidomics of Ixodes scapularis synganglion. Journal of Proteomics. 2009 Aug 20;72(6):1040-1045. https://doi.org/10.1016/j.jprot.2009.06.007
Neupert, Susanne ; Russell, William ; Predel, Reinhard ; Russell, David H. ; Strey, Otto F. ; Teel, Pete D. ; Nachman, Ronald J. / The neuropeptidomics of Ixodes scapularis synganglion. In: Journal of Proteomics. 2009 ; Vol. 72, No. 6. pp. 1040-1045.
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