The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation

Sungsu Kim, Yogesh Wairkar, Richard W. Daniels, Aaron DiAntonio

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Endosomal maturation is critical for accurate and efficient cargo transport through endosomal compartments. Here we identify a mutation of the novel Drosophila gene, ema (endosomal maturation defective) in a screen for abnormal synaptic overgrowth and defective protein trafficking. Ema is an endosomal membrane protein required for trafficking of fluid-phase and receptor-mediated endocytic cargos. In the ema mutant, enlarged endosomal compartments accumulate as endosomal maturation fails, with early and late endosomes unable to progress into mature degradative late endosomes and lysosomes. Defective endosomal downregulation of BMP signaling is responsible for the abnormal synaptic overgrowth. Ema binds to and genetically interacts with Vps16A, a component of the class C Vps-HOPS complex that promotes endosomal maturation. The human orthologue of ema, Clec16A, is a candidate susceptibility locus for autoimmune disorders, and its expression rescues the Drosophila mutant demonstrating conserved function. Characterizing this novel gene family identifies a new component of the endosomal pathway and provides insights into class C Vps-HOPS complex function.

Original languageEnglish (US)
Pages (from-to)717-734
Number of pages18
JournalJournal of Cell Biology
Volume188
Issue number5
DOIs
StatePublished - Mar 8 2010
Externally publishedYes

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Endosomes
Protein Transport
Drosophila
Membrane Proteins
Lysosomes
Genes
Down-Regulation
Mutation

ASJC Scopus subject areas

  • Cell Biology

Cite this

The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. / Kim, Sungsu; Wairkar, Yogesh; Daniels, Richard W.; DiAntonio, Aaron.

In: Journal of Cell Biology, Vol. 188, No. 5, 08.03.2010, p. 717-734.

Research output: Contribution to journalArticle

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