The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae: A potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region

Paul Reynolds, David R. Higgins, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair

Original languageEnglish (US)
Pages (from-to)2357-2372
Number of pages16
JournalNucleic Acids Research
Volume13
Issue number7
DOIs
StatePublished - Apr 11 1985
Externally publishedYes

Fingerprint

Adenine Nucleotides
Saccharomyces Cerevisiae
Amino Acid Sequence
Nucleotides
Yeast
Saccharomyces cerevisiae
Amino acids
Genes
Gene
Proteins
Protein
Amino Acids
Pyrimidine Dimers
Viability
DNA Repair
Open Reading Frames
Carrier Proteins
Adenosine Triphosphate
Molecular Weight
Adenosinetriphosphate

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

@article{b340b40ce65147b88b04b1110e265392,
title = "The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae: A potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region",
abstract = "The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair",
author = "Paul Reynolds and Higgins, {David R.} and Louise Prakash and Satya Prakash",
year = "1985",
month = "4",
day = "11",
doi = "10.1093/nar/13.7.2357",
language = "English (US)",
volume = "13",
pages = "2357--2372",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - The nucleotide sequence of the RAD3 gene of Saccharomyces cerevisiae

T2 - A potential adenine nucleotide binding amino acid sequence and a nonessential acidic carboxyl terminal region

AU - Reynolds, Paul

AU - Higgins, David R.

AU - Prakash, Louise

AU - Prakash, Satya

PY - 1985/4/11

Y1 - 1985/4/11

N2 - The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair

AB - The RAD3 gene of Saccharomyces cerevisiae is required for excision of pyrimidine dimers and is essential for viability. We present the nucleotide sequence of the RAD3 protein coding region and its flanking regions, and the deduced primary structure of the RAD3 protein. In addition, we have mapped the 5′ end of RAD3 mRNA. The predicted RAD3 protein contains 778 amino acids with a calculated molecular weight of 89, 779. A segment of the RAD3 protein shares homology with several adenine nucleotide binding proteins, suggesting that RAD3 protein may react with ATP. The twenty carboxyl terminal amino acids of RAD3 protein are predominantly acidic; however, deletion of this acidic region has no obvious effect on viability or DNA repair

UR - http://www.scopus.com/inward/record.url?scp=0022423251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022423251&partnerID=8YFLogxK

U2 - 10.1093/nar/13.7.2357

DO - 10.1093/nar/13.7.2357

M3 - Article

C2 - 2987851

AN - SCOPUS:0022423251

VL - 13

SP - 2357

EP - 2372

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

ER -