The optimal binding sequence of the Hox11 protein contains a predicted recognition core motif

Shao-Jun Tang, Martin L. Breitman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

HOX11 is a homeobox-contalnlng oncogene of specific T-cell leukemias. We determined the DNA binding specificity of the Hox11 protein by using a novel technique of random ollgonucleotide selection developed in this study. The optimal Hox11 binding sequence, GGCGGTAAGTGG, contained a core TAAGTG motif that Is consistent with a prediction based on the residues at specific positions that potentially make DNA base contacts and models of homeo-domain-DNA interaction proposed from studies with other homeodomains. The specific interaction between Hox11 and the selected optimal binding sequence was further confirmed by band-shift and DNA competition assays. Given that the Hox11 homeodomain shares low homology with other well studied homeodomains, the presence of a predictable recognition core motif in its optimal binding sequence supports the notion that different homeodomains interact with DNA In a similar manner, through highly conserved residues at specific positions that allow contact with DNA.

Original languageEnglish (US)
Pages (from-to)1928-1935
Number of pages8
JournalNucleic Acids Research
Volume23
Issue number11
DOIs
StatePublished - Jun 11 1995
Externally publishedYes

Fingerprint

DNA
Proteins
Protein
Contact
Leukemia
T-cells
Interaction
Specificity
T-Cell Leukemia
Homology
Homeobox Genes
Oncogenes
Prediction
Assays
Model

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The optimal binding sequence of the Hox11 protein contains a predicted recognition core motif. / Tang, Shao-Jun; Breitman, Martin L.

In: Nucleic Acids Research, Vol. 23, No. 11, 11.06.1995, p. 1928-1935.

Research output: Contribution to journalArticle

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