The origin of bone formed by heterotopic periosteal autografts

Taiichi Nishimura, David J. Simmons, Elgene G. Mainous

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Purpose: This study tested the hypothesis that a significant amount of the new bone produced by heterotopic periosteal autografts is derived osteoinductively because proliferating periosteal cells express the bone morphogenetic protein (BMP). Materials and Methods: Rabbit ulnar and radial periosteum were autografted as free grafts (FGs) to the forelimb musculature, and as millipore diffusion chambers grafts (MDCGs) to the rectus abdominus muscle. The grafts were recovered at 3, 5, 7, 14, and 28 days postoperation, fixed in 4% paraformaldehyde, demineralized in 0.6N HCL, and 4.0 μm paraffin-embedded sections were immunostained with monoclonal antibody against recombinant human (rh) BMP-2. Results: Sections from FGs recovered 5 to 28 days postoperatively exhibited cartilage and bone; fibrous tissue, cartilage, bone, and osteochondroid differentiated within MDCGs. Although BMP-2 was expressed by mesenchymal cells, osteoblasts, osteocytes, and osteoclasts, none of the MDCGs produced the osteoinductive signature of transmembrane bone formation. Conclusions: These observations indicated that the larger fraction of the new bone produced by heterotopic periosteal autografts is derived from the graft cells.

    Original languageEnglish (US)
    Pages (from-to)1265-1268
    Number of pages4
    JournalJournal of Oral and Maxillofacial Surgery
    Volume55
    Issue number11
    DOIs
    StatePublished - Nov 1997

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    Autografts
    Transplants
    Bone and Bones
    Cartilage
    Osteocytes
    Bone Morphogenetic Protein 2
    Rectus Abdominis
    Periosteum
    Bone Morphogenetic Proteins
    Forelimb
    Osteoclasts
    Osteoblasts
    Osteogenesis
    Paraffin
    Monoclonal Antibodies
    Rabbits
    Muscles

    ASJC Scopus subject areas

    • Dentistry(all)
    • Surgery

    Cite this

    The origin of bone formed by heterotopic periosteal autografts. / Nishimura, Taiichi; Simmons, David J.; Mainous, Elgene G.

    In: Journal of Oral and Maxillofacial Surgery, Vol. 55, No. 11, 11.1997, p. 1265-1268.

    Research output: Contribution to journalArticle

    Nishimura, Taiichi ; Simmons, David J. ; Mainous, Elgene G. / The origin of bone formed by heterotopic periosteal autografts. In: Journal of Oral and Maxillofacial Surgery. 1997 ; Vol. 55, No. 11. pp. 1265-1268.
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