The other estrogen receptor in the plasma membrane: Implications for the actions of environmental estrogens

C. S. Watson, T. C. Pappas, B. Gametchu

    Research output: Contribution to journalArticle

    85 Citations (Scopus)

    Abstract

    Environmental or nutritional estrogenic toxicants are thought to mediate developmental and carcinogenic pathologies. Estrogen receptor (ER) measurements are currently used to predict hormonal responsiveness; therefore all ER subpopulations should be considered. We have been involved in the immunoidentification and characterization of membrane steroid receptors in several systems and have recently shown that binding of estradiol (E2) to a subpopulation of ERs (mER) residing in the plasma membrane of GH3 pituitary tumor cells mediates the rapid release of prolactin (PRL). Here we review these findings and present other important characterizations of these receptors such as trypsin and serum susceptibility, movement in the membrane, confocal localization to the membrane, binding to and function of impeded ligands, and immunoseparation of cells bearing mER. We plan to use this system as a model for both the physiological and pathological nongenomic effects of estrogens and estrogenic xenobiotics. Specifically, it should be useful as an in vitro assay system for the ability of estrogenic xenobiotics to cause rapid PRL release as an example of nongenomic estrogen effects.

    Original languageEnglish (US)
    Pages (from-to)41-50
    Number of pages10
    JournalEnvironmental Health Perspectives
    Volume103
    Issue numberSUPPL. 7
    StatePublished - 1995

    Fingerprint

    Cell membranes
    Estrogen Receptors
    Estrogens
    Cell Membrane
    Xenobiotics
    membrane
    Membranes
    plasma
    Prolactin
    xenobiotics
    Bearings (structural)
    subpopulation
    Steroid Receptors
    Pituitary Neoplasms
    Pathology
    Trypsin
    Tumors
    Estradiol
    Assays
    pathology

    Keywords

    • Estrogen
    • Nongenomic
    • Pituitary
    • Plasma membrane
    • Prolactin
    • Steroid receptor
    • Xenobiotics

    ASJC Scopus subject areas

    • Environmental Science(all)
    • Environmental Chemistry
    • Public Health, Environmental and Occupational Health

    Cite this

    The other estrogen receptor in the plasma membrane : Implications for the actions of environmental estrogens. / Watson, C. S.; Pappas, T. C.; Gametchu, B.

    In: Environmental Health Perspectives, Vol. 103, No. SUPPL. 7, 1995, p. 41-50.

    Research output: Contribution to journalArticle

    Watson, C. S. ; Pappas, T. C. ; Gametchu, B. / The other estrogen receptor in the plasma membrane : Implications for the actions of environmental estrogens. In: Environmental Health Perspectives. 1995 ; Vol. 103, No. SUPPL. 7. pp. 41-50.
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