The Panton–Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300

E.L. Brown, O. Dumitrescu, D. Thomas, C. Badiou, E.M. Koers, P. Choudhury, Vanessa Vazquez, J. Etienne, G. Lina, F. Vandenesch, M.G. Bowden

Research output: Contribution to journalArticle

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Abstract

Methicillin-resistant Staphylococcus aureus is increasingly responsible for staphylococcal infections in the community. A large percentage of the community-acquired methicillin-resistant (CA-MRSA) strains in the USA produce Panton–Valentine leukocidin (PVL), which is associated with severe infections. The virulence of the clinical CA-MRSA strain USA300 was compared to that of its isogenic pvl-deleted mutant, and it was shown that PVL contributes to lung and muscle tissue destruction, respectively, in murine necrotizing pneumonia and skin infection models. Mice infected with the USA300 strain developed a dominant anti-PVL response. The PVL subunits were therefore tested as vaccinogens against this isolate, and their vaccine efficacy correlated with both the route of vaccination and infection. These data suggest that PVL is a virulence factor in murine CA-MRSA infections.
Original languageEnglish (US)
Pages (from-to)156-164
JournalClinical Microbiology and Infection
Volume15
Issue number2
StatePublished - 2009
Externally publishedYes

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Leukocidins
Staphylococcus aureus
Vaccines
Methicillin Resistance
Lung
Skin
Infection
Staphylococcal Infections
Virulence Factors
Methicillin-Resistant Staphylococcus aureus
Virulence
Vaccination
Muscles

Cite this

Brown, E. L., Dumitrescu, O., Thomas, D., Badiou, C., Koers, E. M., Choudhury, P., ... Bowden, M. G. (2009). The Panton–Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300. Clinical Microbiology and Infection, 15(2), 156-164.

The Panton–Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300. / Brown, E.L.; Dumitrescu, O.; Thomas, D.; Badiou, C.; Koers, E.M.; Choudhury, P.; Vazquez, Vanessa; Etienne, J. ; Lina, G. ; Vandenesch, F.; Bowden, M.G.

In: Clinical Microbiology and Infection, Vol. 15, No. 2, 2009, p. 156-164.

Research output: Contribution to journalArticle

Brown, EL, Dumitrescu, O, Thomas, D, Badiou, C, Koers, EM, Choudhury, P, Vazquez, V, Etienne, J, Lina, G, Vandenesch, F & Bowden, MG 2009, 'The Panton–Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300', Clinical Microbiology and Infection, vol. 15, no. 2, pp. 156-164.
Brown, E.L. ; Dumitrescu, O. ; Thomas, D. ; Badiou, C. ; Koers, E.M. ; Choudhury, P. ; Vazquez, Vanessa ; Etienne, J. ; Lina, G. ; Vandenesch, F. ; Bowden, M.G. / The Panton–Valentine leukocidin vaccine protects mice against lung and skin infections caused by Staphylococcus aureus USA300. In: Clinical Microbiology and Infection. 2009 ; Vol. 15, No. 2. pp. 156-164.
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AB - Methicillin-resistant Staphylococcus aureus is increasingly responsible for staphylococcal infections in the community. A large percentage of the community-acquired methicillin-resistant (CA-MRSA) strains in the USA produce Panton–Valentine leukocidin (PVL), which is associated with severe infections. The virulence of the clinical CA-MRSA strain USA300 was compared to that of its isogenic pvl-deleted mutant, and it was shown that PVL contributes to lung and muscle tissue destruction, respectively, in murine necrotizing pneumonia and skin infection models. Mice infected with the USA300 strain developed a dominant anti-PVL response. The PVL subunits were therefore tested as vaccinogens against this isolate, and their vaccine efficacy correlated with both the route of vaccination and infection. These data suggest that PVL is a virulence factor in murine CA-MRSA infections.

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