The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis

Krishna Moorthi Bhat

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The Drosophila signaling molecule Wingless (Wg) plays crucial roles in cell-cell communications during development. In the developing nervous system, a previous study has shown that Wg acts non-autonomously to specify the fate of a specific neuronal precursor, NB4-2. The lack of autocrine specification of NB4-2 in Wg-expressing cells suggests that the response to Wg is spatially restricted, presumably through the activity of the Wg-receptor. I show that two other proteins, a transcription factor Gooseberry (Gsb) and a transmembrane protein Patched (Ptc), participate in the Wg-mediated specification of NB4-2 by controlling the response to the Wg signal. In gsb mutants, Wg-positive NB5-3 is transformed to NB4-2 in a Wg-dependent manner, suggesting that Gsb normally represses the capacity to respond to the Wg signal. In ptc mutants, Gsb is ectopically expressed in normally Wg-responsive cells, thus preventing the Wg response and consequently the correct specification of NB4-2 does not take place. This conclusion is supported by the observation that NB4-2 can be specified in gsb;ptc double mutants in a Wg-dependent manner. Moreover, ectopic expression of Gsb from the hsp70-gsb transgene also blocks the response to the Wg signal. I propose that the responsiveness to the Wg signal is controlled by sequential negative regulation, ptc→gsb→Wg receptor. The timing of the response to Gsb suggests that the specification of neuroblast identities takes place within the neuroectoderm, prior to neuroblast delamination.

Original languageEnglish (US)
Pages (from-to)2921-2932
Number of pages12
JournalDevelopment
Volume122
Issue number9
StatePublished - 1996
Externally publishedYes

Fingerprint

Ribes
Neurogenesis
Drosophila
Neural Plate
Transgenes
Cell Communication
Nervous System
Transcription Factors
Proteins

Keywords

  • Gooseberry
  • Neuroblast
  • Neurogenesis
  • Patched
  • Signaling
  • Wingless

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

The patched signaling pathway mediates repression of gooseberry allowing neuroblast specification by wingless during Drosophila neurogenesis. / Bhat, Krishna Moorthi.

In: Development, Vol. 122, No. 9, 1996, p. 2921-2932.

Research output: Contribution to journalArticle

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