The peroxidatic and catalatic activity of catalase in normal and acatalasemic mouse liver

Satish K. Srivastava, Naseem H. Ansari

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Monomeric, dimeric and tetrameric forms of mouse liver catalase have been shown to express peroxidatic activity while the tetrameric form expresses the catalic activity. Autosomally inherited acatalasemia, produced by X-ray irradiation of mice results in almost complete loss of catalic activity of catalase but has no effect on the peroxidatic activity. Liver catalase from normal and acatalasemic mice was purified by following the catalic and peroxidatic activity, respectively. Antiserum produced in rabbit against catalase from normal mouse completely precipitated the catalatic and peroxidatic activity from normal liver, and peroxidatic activity from the acatalasemic liver homogenate. Similar results were obtained when antiserum against peroxidase from acatalasemic mice was used. These studies indicate that acatalasemia in mice is due to a structural gene mutation which leads to synthesis of structurally altered catalase subunits. The altered subunits express peroxidatic activity but do not combine to form a tetramer which expresses catalatic activity.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalBBA - General Subjects
Volume633
Issue number3
DOIs
StatePublished - Dec 15 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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