The peroxidatic and catalatic activity of catalase in normal and acatalasemic mouse liver

Satish Srivastava, Naseem Ansari

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Monomeric, dimeric and tetrameric forms of mouse liver catalase have been shown to express peroxidatic activity while the tetrameric form expresses the catalic activity. Autosomally inherited acatalasemia, produced by X-ray irradiation of mice results in almost complete loss of catalic activity of catalase but has no effect on the peroxidatic activity. Liver catalase from normal and acatalasemic mice was purified by following the catalic and peroxidatic activity, respectively. Antiserum produced in rabbit against catalase from normal mouse completely precipitated the catalatic and peroxidatic activity from normal liver, and peroxidatic activity from the acatalasemic liver homogenate. Similar results were obtained when antiserum against peroxidase from acatalasemic mice was used. These studies indicate that acatalasemia in mice is due to a structural gene mutation which leads to synthesis of structurally altered catalase subunits. The altered subunits express peroxidatic activity but do not combine to form a tetramer which expresses catalatic activity.

Original languageEnglish (US)
Pages (from-to)317-322
Number of pages6
JournalBBA - General Subjects
Volume633
Issue number3
DOIs
StatePublished - Dec 15 1980
Externally publishedYes

Fingerprint

Liver
Catalase
Acatalasia
Immune Sera
Peroxidase
Genes
Irradiation
X rays
X-Rays
Rabbits
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Medicine(all)

Cite this

The peroxidatic and catalatic activity of catalase in normal and acatalasemic mouse liver. / Srivastava, Satish; Ansari, Naseem.

In: BBA - General Subjects, Vol. 633, No. 3, 15.12.1980, p. 317-322.

Research output: Contribution to journalArticle

@article{f124d9b7b4a34ec1bb9b8ed754e6c5bc,
title = "The peroxidatic and catalatic activity of catalase in normal and acatalasemic mouse liver",
abstract = "Monomeric, dimeric and tetrameric forms of mouse liver catalase have been shown to express peroxidatic activity while the tetrameric form expresses the catalic activity. Autosomally inherited acatalasemia, produced by X-ray irradiation of mice results in almost complete loss of catalic activity of catalase but has no effect on the peroxidatic activity. Liver catalase from normal and acatalasemic mice was purified by following the catalic and peroxidatic activity, respectively. Antiserum produced in rabbit against catalase from normal mouse completely precipitated the catalatic and peroxidatic activity from normal liver, and peroxidatic activity from the acatalasemic liver homogenate. Similar results were obtained when antiserum against peroxidase from acatalasemic mice was used. These studies indicate that acatalasemia in mice is due to a structural gene mutation which leads to synthesis of structurally altered catalase subunits. The altered subunits express peroxidatic activity but do not combine to form a tetramer which expresses catalatic activity.",
author = "Satish Srivastava and Naseem Ansari",
year = "1980",
month = "12",
day = "15",
doi = "10.1016/0304-4165(80)90191-9",
language = "English (US)",
volume = "633",
pages = "317--322",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - The peroxidatic and catalatic activity of catalase in normal and acatalasemic mouse liver

AU - Srivastava, Satish

AU - Ansari, Naseem

PY - 1980/12/15

Y1 - 1980/12/15

N2 - Monomeric, dimeric and tetrameric forms of mouse liver catalase have been shown to express peroxidatic activity while the tetrameric form expresses the catalic activity. Autosomally inherited acatalasemia, produced by X-ray irradiation of mice results in almost complete loss of catalic activity of catalase but has no effect on the peroxidatic activity. Liver catalase from normal and acatalasemic mice was purified by following the catalic and peroxidatic activity, respectively. Antiserum produced in rabbit against catalase from normal mouse completely precipitated the catalatic and peroxidatic activity from normal liver, and peroxidatic activity from the acatalasemic liver homogenate. Similar results were obtained when antiserum against peroxidase from acatalasemic mice was used. These studies indicate that acatalasemia in mice is due to a structural gene mutation which leads to synthesis of structurally altered catalase subunits. The altered subunits express peroxidatic activity but do not combine to form a tetramer which expresses catalatic activity.

AB - Monomeric, dimeric and tetrameric forms of mouse liver catalase have been shown to express peroxidatic activity while the tetrameric form expresses the catalic activity. Autosomally inherited acatalasemia, produced by X-ray irradiation of mice results in almost complete loss of catalic activity of catalase but has no effect on the peroxidatic activity. Liver catalase from normal and acatalasemic mice was purified by following the catalic and peroxidatic activity, respectively. Antiserum produced in rabbit against catalase from normal mouse completely precipitated the catalatic and peroxidatic activity from normal liver, and peroxidatic activity from the acatalasemic liver homogenate. Similar results were obtained when antiserum against peroxidase from acatalasemic mice was used. These studies indicate that acatalasemia in mice is due to a structural gene mutation which leads to synthesis of structurally altered catalase subunits. The altered subunits express peroxidatic activity but do not combine to form a tetramer which expresses catalatic activity.

UR - http://www.scopus.com/inward/record.url?scp=0019295517&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019295517&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(80)90191-9

DO - 10.1016/0304-4165(80)90191-9

M3 - Article

C2 - 7213667

AN - SCOPUS:0019295517

VL - 633

SP - 317

EP - 322

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 3

ER -