The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock

Dirk M. Maybauer, Marc O. Maybauer, Csaba Szabo, Martin Westphal, Lillian D. Traber, Andrew L. Salzman, David Herndon, Daniel L. Traber

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

This prospective, randomized, controlled experimental study examined the effects of the peroxynitrite decomposition catalyst WW-85 on global hemodynamics and regional microvascular blood flow (RMBF) in an established ovine model of septic shock following severe smoke inhalation injury. Twenty-one sheep were randomized into a sham group (no injury), a control group (smoke/sepsis), and a treatment group (smoke/sepsis/WW-85; n = 7 each). WW-85 was administered 1 h after injury as a bolus (0.1 mg/kg), followed by a continuous infusion of 0.02 mg/kg/h RMBF was analyzed using colored microspheres. All control animals developed a hypotensive, hyperdynamic circulation and increased plasma levels of nitrate/-nitrite (NOx). All hemodynamic variables and NOx levels were significantly improved in the treatment group. In visceral organs of controls, blood flow to trachea, ileum, and spleen significantly increased (p < 0.05). Blood flow to kidneys and pancreas significantly decreased (p < 0.05). Treatment with WW-85 stabilized blood flow to ileum, spleen, and kidneys on baseline levels and was significantly improved compared to controls (p < 0.05). Cerebral blood flow deteriorated in controls, but was significantly improved in cerebral cortex, cerebellum, pons, medulla oblongata, and thalamus (p < 0.05) by WW-85. These results provide evidence that WW-85 blocks NO production, thereby improving cardiovascular function and microcirculation.

Original languageEnglish (US)
Pages (from-to)841-849
Number of pages9
JournalBurns
Volume37
Issue number5
DOIs
StatePublished - Aug 2011

Fingerprint

Smoke Inhalation Injury
Peroxynitrous Acid
Microcirculation
Septic Shock
Sheep
Regional Blood Flow
Ileum
Smoke
Sepsis
Cerebrovascular Circulation
Spleen
Hemodynamics
Kidney
Medulla Oblongata
Pons
Wounds and Injuries
Nitrites
Trachea
Thalamus
Microspheres

Keywords

  • Acute lung injury
  • ARDS
  • Cerebral autoregulation
  • Cerebral blood flow
  • Nitric oxide
  • Smoke inhalation

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine
  • Surgery

Cite this

Maybauer, D. M., Maybauer, M. O., Szabo, C., Westphal, M., Traber, L. D., Salzman, A. L., ... Traber, D. L. (2011). The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock. Burns, 37(5), 841-849. https://doi.org/10.1016/j.burns.2010.12.020

The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock. / Maybauer, Dirk M.; Maybauer, Marc O.; Szabo, Csaba; Westphal, Martin; Traber, Lillian D.; Salzman, Andrew L.; Herndon, David; Traber, Daniel L.

In: Burns, Vol. 37, No. 5, 08.2011, p. 841-849.

Research output: Contribution to journalArticle

Maybauer, DM, Maybauer, MO, Szabo, C, Westphal, M, Traber, LD, Salzman, AL, Herndon, D & Traber, DL 2011, 'The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock', Burns, vol. 37, no. 5, pp. 841-849. https://doi.org/10.1016/j.burns.2010.12.020
Maybauer, Dirk M. ; Maybauer, Marc O. ; Szabo, Csaba ; Westphal, Martin ; Traber, Lillian D. ; Salzman, Andrew L. ; Herndon, David ; Traber, Daniel L. / The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock. In: Burns. 2011 ; Vol. 37, No. 5. pp. 841-849.
@article{707260a71aab49758424785fee827a8c,
title = "The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock",
abstract = "This prospective, randomized, controlled experimental study examined the effects of the peroxynitrite decomposition catalyst WW-85 on global hemodynamics and regional microvascular blood flow (RMBF) in an established ovine model of septic shock following severe smoke inhalation injury. Twenty-one sheep were randomized into a sham group (no injury), a control group (smoke/sepsis), and a treatment group (smoke/sepsis/WW-85; n = 7 each). WW-85 was administered 1 h after injury as a bolus (0.1 mg/kg), followed by a continuous infusion of 0.02 mg/kg/h RMBF was analyzed using colored microspheres. All control animals developed a hypotensive, hyperdynamic circulation and increased plasma levels of nitrate/-nitrite (NOx). All hemodynamic variables and NOx levels were significantly improved in the treatment group. In visceral organs of controls, blood flow to trachea, ileum, and spleen significantly increased (p < 0.05). Blood flow to kidneys and pancreas significantly decreased (p < 0.05). Treatment with WW-85 stabilized blood flow to ileum, spleen, and kidneys on baseline levels and was significantly improved compared to controls (p < 0.05). Cerebral blood flow deteriorated in controls, but was significantly improved in cerebral cortex, cerebellum, pons, medulla oblongata, and thalamus (p < 0.05) by WW-85. These results provide evidence that WW-85 blocks NO production, thereby improving cardiovascular function and microcirculation.",
keywords = "Acute lung injury, ARDS, Cerebral autoregulation, Cerebral blood flow, Nitric oxide, Smoke inhalation",
author = "Maybauer, {Dirk M.} and Maybauer, {Marc O.} and Csaba Szabo and Martin Westphal and Traber, {Lillian D.} and Salzman, {Andrew L.} and David Herndon and Traber, {Daniel L.}",
year = "2011",
month = "8",
doi = "10.1016/j.burns.2010.12.020",
language = "English (US)",
volume = "37",
pages = "841--849",
journal = "Burns",
issn = "0305-4179",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - The peroxynitrite catalyst WW-85 improves microcirculation in ovine smoke inhalation injury and septic shock

AU - Maybauer, Dirk M.

AU - Maybauer, Marc O.

AU - Szabo, Csaba

AU - Westphal, Martin

AU - Traber, Lillian D.

AU - Salzman, Andrew L.

AU - Herndon, David

AU - Traber, Daniel L.

PY - 2011/8

Y1 - 2011/8

N2 - This prospective, randomized, controlled experimental study examined the effects of the peroxynitrite decomposition catalyst WW-85 on global hemodynamics and regional microvascular blood flow (RMBF) in an established ovine model of septic shock following severe smoke inhalation injury. Twenty-one sheep were randomized into a sham group (no injury), a control group (smoke/sepsis), and a treatment group (smoke/sepsis/WW-85; n = 7 each). WW-85 was administered 1 h after injury as a bolus (0.1 mg/kg), followed by a continuous infusion of 0.02 mg/kg/h RMBF was analyzed using colored microspheres. All control animals developed a hypotensive, hyperdynamic circulation and increased plasma levels of nitrate/-nitrite (NOx). All hemodynamic variables and NOx levels were significantly improved in the treatment group. In visceral organs of controls, blood flow to trachea, ileum, and spleen significantly increased (p < 0.05). Blood flow to kidneys and pancreas significantly decreased (p < 0.05). Treatment with WW-85 stabilized blood flow to ileum, spleen, and kidneys on baseline levels and was significantly improved compared to controls (p < 0.05). Cerebral blood flow deteriorated in controls, but was significantly improved in cerebral cortex, cerebellum, pons, medulla oblongata, and thalamus (p < 0.05) by WW-85. These results provide evidence that WW-85 blocks NO production, thereby improving cardiovascular function and microcirculation.

AB - This prospective, randomized, controlled experimental study examined the effects of the peroxynitrite decomposition catalyst WW-85 on global hemodynamics and regional microvascular blood flow (RMBF) in an established ovine model of septic shock following severe smoke inhalation injury. Twenty-one sheep were randomized into a sham group (no injury), a control group (smoke/sepsis), and a treatment group (smoke/sepsis/WW-85; n = 7 each). WW-85 was administered 1 h after injury as a bolus (0.1 mg/kg), followed by a continuous infusion of 0.02 mg/kg/h RMBF was analyzed using colored microspheres. All control animals developed a hypotensive, hyperdynamic circulation and increased plasma levels of nitrate/-nitrite (NOx). All hemodynamic variables and NOx levels were significantly improved in the treatment group. In visceral organs of controls, blood flow to trachea, ileum, and spleen significantly increased (p < 0.05). Blood flow to kidneys and pancreas significantly decreased (p < 0.05). Treatment with WW-85 stabilized blood flow to ileum, spleen, and kidneys on baseline levels and was significantly improved compared to controls (p < 0.05). Cerebral blood flow deteriorated in controls, but was significantly improved in cerebral cortex, cerebellum, pons, medulla oblongata, and thalamus (p < 0.05) by WW-85. These results provide evidence that WW-85 blocks NO production, thereby improving cardiovascular function and microcirculation.

KW - Acute lung injury

KW - ARDS

KW - Cerebral autoregulation

KW - Cerebral blood flow

KW - Nitric oxide

KW - Smoke inhalation

UR - http://www.scopus.com/inward/record.url?scp=79960410491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960410491&partnerID=8YFLogxK

U2 - 10.1016/j.burns.2010.12.020

DO - 10.1016/j.burns.2010.12.020

M3 - Article

C2 - 21345593

AN - SCOPUS:79960410491

VL - 37

SP - 841

EP - 849

JO - Burns

JF - Burns

SN - 0305-4179

IS - 5

ER -