The polypyrimidine tract-binding protein is required for efficient dengue virus propagation and associates with the viral replication machinery

Azlinda Anwar, K. M. Leong, Mary L. Ng, Justin J H Chu, Mariano Garcia-Blanco

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The polypyrimidine tract-binding protein (PTB) functions primarily as an IRES trans-acting factor in the propagation of hepatitisCvirus and picornaviruses. PTB interacts with secondary structures within the 3′- and 5′-untranslated regions of these viral genomes to mediate efficient IRES-mediated viral translation. PTB has also been reported to bind to the untranslated region of the single-stranded RNA dengue virus (DENV), suggesting a similar function for PTB in flaviviruses. Indeed small interfering RNA-mediated PTB knockdown inhibited the production of infectious DENV, and this inhibition was specific to PTB knockdown and not due to a nonspecific anti-viral state. In fact, PTB depletion did not inhibit the production infectious yellow fever virus, another flavivirus. Nevertheless, whereas PTB knockdown led to a significant decrease in the accumulation of DENV viral RNAs, it did not impair translation. Moreover, PTB was shown to interact with the DENV nonstructural 4A protein, a known component of the viral replication complex, and with the DENV genome during infection. These data suggest that PTB interacts with the replication complex of DENV and is acting at the level of viral RNA replication.

Original languageEnglish (US)
Pages (from-to)17021-17029
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number25
DOIs
StatePublished - Jun 19 2009
Externally publishedYes

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Polypyrimidine Tract-Binding Protein
Dengue Virus
Viruses
Machinery
Flavivirus
Viral RNA
Genes
Yellow fever virus
Picornaviridae
Untranslated Regions
Viral Structures
Trans-Activators
Viral Genome
5' Untranslated Regions
RNA Viruses
3' Untranslated Regions
Small Interfering RNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The polypyrimidine tract-binding protein is required for efficient dengue virus propagation and associates with the viral replication machinery. / Anwar, Azlinda; Leong, K. M.; Ng, Mary L.; Chu, Justin J H; Garcia-Blanco, Mariano.

In: Journal of Biological Chemistry, Vol. 284, No. 25, 19.06.2009, p. 17021-17029.

Research output: Contribution to journalArticle

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