The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during drosophila oogenesis

Krishna Moorthi Bhat

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

In a variety of tissues in eukaryotes, multipotential stem cells are responsible for maintaining a germinal population and generating a differentiated progeny. The Drosophila germline is one such tissue where a continuous supply of eggs or sperm relies on the normal functioning of stem cells. Recent studies have implicated a possible role for the posterior determinant gene nanos (nos) in stem cells. Here, I report that nanos is required in the Drosophila female germline as well as in the male germline. In the female, nos is required for the functioning of stem cells. In nos mutants, while the stem cells are specified, these cells divide only a few times at the most and then degenerate. The loss of germline stem cells in nos mutant mothers appears to be due to a progressive degeneration of the plasma membrane. Furthermore, following germ cell loss, the germaria in the nos mutant mothers appear to carry on massive mitochondrial biogenesis activity. Thus, the syncytia of such germaria are filled with mitochondria. In the male germline, the male fertility assay indicates that nos appears to be also required for the maintenance of stem cells. In these mutant males, spermatogenesis is progressively affected and these males eventually become sterile. These results indicate novel requirements for nos in the Drosophila germline.

Original languageEnglish (US)
Pages (from-to)1479-1492
Number of pages14
JournalGenetics
Volume151
Issue number4
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Oogenesis
Drosophila
Stem Cells
Maintenance
Genes
Organelle Biogenesis
Giant Cells
Spermatogenesis
Adult Germline Stem Cells
Eukaryota
Germ Cells
Eggs
Fertility
Spermatozoa
Mitochondria
Cell Membrane
Population

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during drosophila oogenesis. / Bhat, Krishna Moorthi.

In: Genetics, Vol. 151, No. 4, 04.1999, p. 1479-1492.

Research output: Contribution to journalArticle

@article{675b7ef398764986949845ab68e562e4,
title = "The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during drosophila oogenesis",
abstract = "In a variety of tissues in eukaryotes, multipotential stem cells are responsible for maintaining a germinal population and generating a differentiated progeny. The Drosophila germline is one such tissue where a continuous supply of eggs or sperm relies on the normal functioning of stem cells. Recent studies have implicated a possible role for the posterior determinant gene nanos (nos) in stem cells. Here, I report that nanos is required in the Drosophila female germline as well as in the male germline. In the female, nos is required for the functioning of stem cells. In nos mutants, while the stem cells are specified, these cells divide only a few times at the most and then degenerate. The loss of germline stem cells in nos mutant mothers appears to be due to a progressive degeneration of the plasma membrane. Furthermore, following germ cell loss, the germaria in the nos mutant mothers appear to carry on massive mitochondrial biogenesis activity. Thus, the syncytia of such germaria are filled with mitochondria. In the male germline, the male fertility assay indicates that nos appears to be also required for the maintenance of stem cells. In these mutant males, spermatogenesis is progressively affected and these males eventually become sterile. These results indicate novel requirements for nos in the Drosophila germline.",
author = "Bhat, {Krishna Moorthi}",
year = "1999",
month = "4",
language = "English (US)",
volume = "151",
pages = "1479--1492",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",

}

TY - JOUR

T1 - The posterior determinant gene nanos is required for the maintenance of the adult germline stem cells during drosophila oogenesis

AU - Bhat, Krishna Moorthi

PY - 1999/4

Y1 - 1999/4

N2 - In a variety of tissues in eukaryotes, multipotential stem cells are responsible for maintaining a germinal population and generating a differentiated progeny. The Drosophila germline is one such tissue where a continuous supply of eggs or sperm relies on the normal functioning of stem cells. Recent studies have implicated a possible role for the posterior determinant gene nanos (nos) in stem cells. Here, I report that nanos is required in the Drosophila female germline as well as in the male germline. In the female, nos is required for the functioning of stem cells. In nos mutants, while the stem cells are specified, these cells divide only a few times at the most and then degenerate. The loss of germline stem cells in nos mutant mothers appears to be due to a progressive degeneration of the plasma membrane. Furthermore, following germ cell loss, the germaria in the nos mutant mothers appear to carry on massive mitochondrial biogenesis activity. Thus, the syncytia of such germaria are filled with mitochondria. In the male germline, the male fertility assay indicates that nos appears to be also required for the maintenance of stem cells. In these mutant males, spermatogenesis is progressively affected and these males eventually become sterile. These results indicate novel requirements for nos in the Drosophila germline.

AB - In a variety of tissues in eukaryotes, multipotential stem cells are responsible for maintaining a germinal population and generating a differentiated progeny. The Drosophila germline is one such tissue where a continuous supply of eggs or sperm relies on the normal functioning of stem cells. Recent studies have implicated a possible role for the posterior determinant gene nanos (nos) in stem cells. Here, I report that nanos is required in the Drosophila female germline as well as in the male germline. In the female, nos is required for the functioning of stem cells. In nos mutants, while the stem cells are specified, these cells divide only a few times at the most and then degenerate. The loss of germline stem cells in nos mutant mothers appears to be due to a progressive degeneration of the plasma membrane. Furthermore, following germ cell loss, the germaria in the nos mutant mothers appear to carry on massive mitochondrial biogenesis activity. Thus, the syncytia of such germaria are filled with mitochondria. In the male germline, the male fertility assay indicates that nos appears to be also required for the maintenance of stem cells. In these mutant males, spermatogenesis is progressively affected and these males eventually become sterile. These results indicate novel requirements for nos in the Drosophila germline.

UR - http://www.scopus.com/inward/record.url?scp=0032920978&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032920978&partnerID=8YFLogxK

M3 - Article

VL - 151

SP - 1479

EP - 1492

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -