The preparation and properties of nerve growth factor protein at alkaline pH

J. Regino Perez Polo, E. M. Shooter

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The nerve growth factor (NGF) subunit of 7S NGF was isolated by chromatography at high pH on QAE Sephadex. It has the same specific NGF activity as βNGF isolated at acid pH, showing that this activity is an intrinsic property of the subunit and is independent of the pathway of dissociation. Continued exposure of the NGF subunit to high pH resulted in an increase in the amount of the minor species β2NGF and the formation of a new species, β3NGF, of even lower isoelectric point. These two species and the original major species of the preparation, β1, were isolated by isoelectric focusing. All three species had the same specific NGF activity, but differed in their ability to reform 7S NGF. The β2 species was one fifth as competent as β1, while β3 was unable to regenerate 7S NGF. Addition of α and γ subunits to β1NGF decreased the amount of NGF protein required to produce one Biological Unit of activity in the bioassay, but had no effect when added to β3NGF. The interactions between the subunits in 7S NGF therefore determine, in part, the specific activity of the NGF subunit.

Original languageEnglish (US)
Pages (from-to)329-338
Number of pages10
JournalNeurobiology
Volume5
Issue number6
StatePublished - 1975
Externally publishedYes

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Nerve Growth Factor
Proteins
Isoelectric Point
Isoelectric Focusing
Biological Assay
Chromatography
Acids

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Regino Perez Polo, J., & Shooter, E. M. (1975). The preparation and properties of nerve growth factor protein at alkaline pH. Neurobiology, 5(6), 329-338.

The preparation and properties of nerve growth factor protein at alkaline pH. / Regino Perez Polo, J.; Shooter, E. M.

In: Neurobiology, Vol. 5, No. 6, 1975, p. 329-338.

Research output: Contribution to journalArticle

Regino Perez Polo, J & Shooter, EM 1975, 'The preparation and properties of nerve growth factor protein at alkaline pH', Neurobiology, vol. 5, no. 6, pp. 329-338.
Regino Perez Polo, J. ; Shooter, E. M. / The preparation and properties of nerve growth factor protein at alkaline pH. In: Neurobiology. 1975 ; Vol. 5, No. 6. pp. 329-338.
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