The process of microbial translocation

J. Wesley Alexander, Steven T. Boyce, George F. Babcock, Luca Gianotti, Michael D. Peck, David L. Dunn, Tonyia Eaves-Pyles, Charles P. Childress, Sara K. Ash

Research output: Contribution to journalArticle

399 Citations (Scopus)

Abstract

The process of microbial translocation was studied using Candida albicans, Escherichia coli, or endotoxin instilled into Thiry-Vella loops of thermally injured guinea pigs and rats. Translocation of C. albicans occurred by direct penetration of enterocytes by a unique process different from classical phagocytosis. Translocation between enterocytes was not observed. Internalization was associated with a disturbance of the plasma membrane and brush border, but most internalized organisms were not surrounded by a plasma membrane. Passage of the Candida into the lamina propria appeared to be associated with disruption of the basal membrane with extrusion of cytoplasm of the cell and Candida. Organisms in the lamina propria were commonly phagocytized by macrophages but also were found free in lymphatics and blood vessels. Translocation of E. coli and endotoxin also occurred directly through enterocytes rather than between them, but translocated endotoxin diffused through the lamina propria and muscular wall of the bowel wall by passing between rather than through the myocytes. These descriptive phenomena provide new insight into the role of the enterocyte and intestinal immune cells in the translocation process.

Original languageEnglish (US)
Pages (from-to)496-510
Number of pages15
JournalAnnals of Surgery
Volume212
Issue number4
StatePublished - Oct 1990
Externally publishedYes

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Enterocytes
Mucous Membrane
Candida albicans
Candida
Cell Membrane
Lymphatic Vessels
Microvilli
Phagocytosis
Endotoxins
Muscle Cells
Blood Vessels
Guinea Pigs
Cytoplasm
Macrophages
Membranes
Escherichia coli endotoxin

ASJC Scopus subject areas

  • Surgery

Cite this

Alexander, J. W., Boyce, S. T., Babcock, G. F., Gianotti, L., Peck, M. D., Dunn, D. L., ... Ash, S. K. (1990). The process of microbial translocation. Annals of Surgery, 212(4), 496-510.

The process of microbial translocation. / Alexander, J. Wesley; Boyce, Steven T.; Babcock, George F.; Gianotti, Luca; Peck, Michael D.; Dunn, David L.; Eaves-Pyles, Tonyia; Childress, Charles P.; Ash, Sara K.

In: Annals of Surgery, Vol. 212, No. 4, 10.1990, p. 496-510.

Research output: Contribution to journalArticle

Alexander, JW, Boyce, ST, Babcock, GF, Gianotti, L, Peck, MD, Dunn, DL, Eaves-Pyles, T, Childress, CP & Ash, SK 1990, 'The process of microbial translocation', Annals of Surgery, vol. 212, no. 4, pp. 496-510.
Alexander JW, Boyce ST, Babcock GF, Gianotti L, Peck MD, Dunn DL et al. The process of microbial translocation. Annals of Surgery. 1990 Oct;212(4):496-510.
Alexander, J. Wesley ; Boyce, Steven T. ; Babcock, George F. ; Gianotti, Luca ; Peck, Michael D. ; Dunn, David L. ; Eaves-Pyles, Tonyia ; Childress, Charles P. ; Ash, Sara K. / The process of microbial translocation. In: Annals of Surgery. 1990 ; Vol. 212, No. 4. pp. 496-510.
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