The protease-sensitive loop of the vesicular stomatitis virus matrix protein is involved in virus assembly and protein translation

Chad Mire, Michael A. Whitt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To study the contribution of the protease-sensitive loop of the VSV M protein in virus assembly we recovered recombinant VSV (rVSV) with mutations in this region and examined virus replication. Mutations in the highly conserved LXD motif (aa 123-125) resulted in reduced virion budding, reduced virus titers and enhanced M protein exchange with M-ribonucleocapsid complexes (M-RNPs), suggesting that the mutant M proteins were less tightly associated with RNP skeletons. In addition, viral protein synthesis began to decrease at 4. h post-infection (hpi) and was reduced by ~. 80% at 8 hpi for the mutant rVSV-D125A. The reduced protein synthesis was not due to decreased VSV replication or transcription; however, translation of a reporter gene with an EMCV IRES was not reduced, suggesting that cap-dependent, but not cap-independent translation initiation was affected in rVSV-D125A infected cells. These results indicate that the LXD motif is involved in both virus assembly and VSV protein translation.

Original languageEnglish (US)
Pages (from-to)16-25
Number of pages10
JournalVirology
Volume416
Issue number1-2
DOIs
StatePublished - Jul 20 2011

Fingerprint

Virus Assembly
Vesicular Stomatitis
Protein Biosynthesis
Peptide Hydrolases
Encephalomyocarditis virus
Viruses
Mutation
Viral Proteins
Mutant Proteins
Virus Replication
Infection
Viral Load
Reporter Genes
Skeleton
Virion
Proteins
Vesicular stomatitis virus M protein

Keywords

  • Matrix protein
  • Vesicular stomatitis virus
  • Virus assembly
  • Virus protein translation

ASJC Scopus subject areas

  • Virology

Cite this

The protease-sensitive loop of the vesicular stomatitis virus matrix protein is involved in virus assembly and protein translation. / Mire, Chad; Whitt, Michael A.

In: Virology, Vol. 416, No. 1-2, 20.07.2011, p. 16-25.

Research output: Contribution to journalArticle

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