The proteinase-activated receptor 2 is involved in nociception

W. A. Hoogerwerf, L. Zou, M. Shenoy, D. Sun, Maria Micci, Helen Hellmich, S. Y. Xiao, John Winston, P. J. Pasricha

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

The proteinase-activated receptor 2 is expressed on a subset of primary afferent neurons and may participate in the neurogenic component of inflammation. We hypothesized that this receptor may also play a role in neuronal sensitization and contribute to the pathogenesis of pain in inflammatory conditions such as pancreatitis. Using a specific proteinase-activated receptor 2 activating peptide, we found evidence of such sensitization in vitro in the form of enhanced capsaicin- and KCl-evoked release of calcitonin gene-related peptide, a marker for nociceptive signaling. We then demonstrated that injection of the proteinase-activated receptor 2 activating peptide into the pancreatic duct can activate and sensitize pancreas-specific afferent neurons in vivo, as measured by Fos expression in the dorsal horn of the spinal cord. These observations suggest that proteinase-activated receptor 2 contributes to nociceptive signaling and may provide a novel link between inflammation and pain.

Original languageEnglish (US)
Pages (from-to)9036-9042
Number of pages7
JournalJournal of Neuroscience
Volume21
Issue number22
StatePublished - Nov 15 2001

Fingerprint

PAR-2 Receptor
Nociception
Afferent Neurons
Neurogenic Inflammation
Pain
Peptides
Calcitonin Gene-Related Peptide
Pancreatic Ducts
Capsaicin
Pancreatitis
Pancreas
Inflammation
Injections

Keywords

  • Dorsal root ganglia
  • Fos
  • Neuronal sensitization
  • Nociception
  • Pancreas
  • Proteinase-activated receptor 2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hoogerwerf, W. A., Zou, L., Shenoy, M., Sun, D., Micci, M., Hellmich, H., ... Pasricha, P. J. (2001). The proteinase-activated receptor 2 is involved in nociception. Journal of Neuroscience, 21(22), 9036-9042.

The proteinase-activated receptor 2 is involved in nociception. / Hoogerwerf, W. A.; Zou, L.; Shenoy, M.; Sun, D.; Micci, Maria; Hellmich, Helen; Xiao, S. Y.; Winston, John; Pasricha, P. J.

In: Journal of Neuroscience, Vol. 21, No. 22, 15.11.2001, p. 9036-9042.

Research output: Contribution to journalArticle

Hoogerwerf, WA, Zou, L, Shenoy, M, Sun, D, Micci, M, Hellmich, H, Xiao, SY, Winston, J & Pasricha, PJ 2001, 'The proteinase-activated receptor 2 is involved in nociception', Journal of Neuroscience, vol. 21, no. 22, pp. 9036-9042.
Hoogerwerf WA, Zou L, Shenoy M, Sun D, Micci M, Hellmich H et al. The proteinase-activated receptor 2 is involved in nociception. Journal of Neuroscience. 2001 Nov 15;21(22):9036-9042.
Hoogerwerf, W. A. ; Zou, L. ; Shenoy, M. ; Sun, D. ; Micci, Maria ; Hellmich, Helen ; Xiao, S. Y. ; Winston, John ; Pasricha, P. J. / The proteinase-activated receptor 2 is involved in nociception. In: Journal of Neuroscience. 2001 ; Vol. 21, No. 22. pp. 9036-9042.
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