The proteomic landscape of glioma stem-like cells

Cheryl F. Lichti, Norelle C. Wildburger, Alexander S. Shavkunov, Ekaterina Mostovenko, Huiling Liu, Erik P. Sulman, Carol L. Nilsson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Glioma stem-like cells (GSCs) are hypothesized to provide a repository of cells in tumors that can self-replicate and are radio- and chemo-resistant. GSC lines, representing several glioma subtypes, have been isolated and characterized at the transcript level. We sought to characterize 35 GSC lines at the protein level using label-free quantitative proteomics. Resulting relative fold changes were used to drive unsupervised hierarchical clustering for the purpose of classifying the cell lines based on proteomic profiles. Bioinformatics analysis identified synoviolin, serine/arginine-rich splicing factor 2, symplekin, and IL-5 as molecules of interest in progression and/or treatment of glioma.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalEuPA Open Proteomics
Volume8
DOIs
StatePublished - Sep 1 2015

Fingerprint

Glioma
Proteomics
Stem Cells
Interleukin-5
Bioinformatics
Serine
Arginine
Labels
Tumors
Cell Line
Cells
Molecules
Proteins
Computational Biology
Radio
Cluster Analysis
Neoplasms

Keywords

  • DAVID
  • Glioblastoma
  • Glioma stem cells
  • Ingenuity Pathway Analysis
  • Label-free quantitative proteomics
  • Unsupervised hierarchical clustering

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lichti, C. F., Wildburger, N. C., Shavkunov, A. S., Mostovenko, E., Liu, H., Sulman, E. P., & Nilsson, C. L. (2015). The proteomic landscape of glioma stem-like cells. EuPA Open Proteomics, 8, 85-93. https://doi.org/10.1016/j.euprot.2015.06.008

The proteomic landscape of glioma stem-like cells. / Lichti, Cheryl F.; Wildburger, Norelle C.; Shavkunov, Alexander S.; Mostovenko, Ekaterina; Liu, Huiling; Sulman, Erik P.; Nilsson, Carol L.

In: EuPA Open Proteomics, Vol. 8, 01.09.2015, p. 85-93.

Research output: Contribution to journalArticle

Lichti, CF, Wildburger, NC, Shavkunov, AS, Mostovenko, E, Liu, H, Sulman, EP & Nilsson, CL 2015, 'The proteomic landscape of glioma stem-like cells', EuPA Open Proteomics, vol. 8, pp. 85-93. https://doi.org/10.1016/j.euprot.2015.06.008
Lichti CF, Wildburger NC, Shavkunov AS, Mostovenko E, Liu H, Sulman EP et al. The proteomic landscape of glioma stem-like cells. EuPA Open Proteomics. 2015 Sep 1;8:85-93. https://doi.org/10.1016/j.euprot.2015.06.008
Lichti, Cheryl F. ; Wildburger, Norelle C. ; Shavkunov, Alexander S. ; Mostovenko, Ekaterina ; Liu, Huiling ; Sulman, Erik P. ; Nilsson, Carol L. / The proteomic landscape of glioma stem-like cells. In: EuPA Open Proteomics. 2015 ; Vol. 8. pp. 85-93.
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