The RAD3 gene of Saccharomyces cerevisiae encodes a DNA-dependent ATPase.

P. Sung, L. Prakash, S. Weber, S. Prakash

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Abstract

The RAD3 gene of the yeast Saccharomyces cerevisiae is required for excision repair of damaged DNA and for cell viability. A protein of approximately equal to 89 kDa was purified to near homogeneity from yeast strains harboring multicopy plasmids that overproduce RAD3 protein; this protein corresponds closely to the expected size of the RAD3 protein and cross-reacts with the antiserum raised against a truncated RAD3 protein produced in Escherichia coli. The purified RAD3 protein shows a single-stranded DNA-dependent ATPase activity that catalyzes hydrolysis of ATP to ADP and Pi. The ATPase activity was coincident with the RAD3 protein during purification and is inhibited by anti-RAD3 antibodies, indicating that the RAD3 gene encodes this activity.

Original languageEnglish (US)
Pages (from-to)6045-6049
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number17
DOIs
StatePublished - Sep 1987
Externally publishedYes

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